Team:UNITN-Trento/Notebook/Labposts/08/04

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{
{
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"date" : "2013-08-01",
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"date" : "2013-08-02",
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"author" : "thomas",
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"author" : "gabriele",
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"title" : "Overnight cultures",
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"title" : "Wintergreen with pLac-SAMsynthetase",
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"content" : "<html>Starting with the plates I did <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-31-thomas\">yesterday</a>, I prepared the following overnight cultures. I did that also with some colonies transformed with pXyl (BBa_K823024) + GFP (BBa_E0840) that Fabio gave me in order to amplify his construct.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Overnight cultures prepared|<html><center><table><tr><th>Plate</th><th>N° of colonies taken</th></tr><tr><td>pXyl + GFP </td><td>3</td></tr><tr><td>pSpac + GFP ligation 1:1 </td><td>2</td></tr><tr><td>pSpac + GFP ligation 1:4</td><td>3</td></tr></table></center></html>}}<html> </html>",
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"content" : "<html>I prepared 3 inocula from both Caterina's plates: 1:3 and 1:4 ligation of pLAC+SAMsynthetase with Wintergreen</html>",
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"tags" : "pSpac-pXyl-GFP"
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"tags" : "Wintergreen-SAMsynthetase-pLac"
}
}

Revision as of 09:02, 3 October 2013

{ "date" : "2013-08-02", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase", "content" : "I prepared 3 inocula from both Caterina's plates: 1:3 and 1:4 ligation of pLAC+SAMsynthetase with Wintergreen", "tags" : "Wintergreen-SAMsynthetase-pLac" }

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