Team:UNITN-Trento/Notebook/Labposts/08/13

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:04, 3 October 2013

{ "date" : "2013-08-06", "author" : "viola", "title" : "3rd ligation", "content" : "today i saw the plates of the cloning that i did yesterday and tha control plate was a mess... there were too many colonies!!i will do some inocula this evening..i try to redo a new ligation adding the SAP after the digestion of EFE in pUC57. Meanwhile i will start a new overnight digestion trying to insert EFE in the PsB1C3 backbone of the part s04617.", "tags" : "s04617-EFE" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-07",
+	"date" : "2013-08-06",
-	"author" : "caterina",
+	"author" : "viola",
-	"title" : "Another biobrick :)",
+	"title" : "3rd ligation",
-	"content" : "<html>I obtained K592016 in the J61002 plasmid under the control of J23100 promoter!I did a screening to confirm the cloning with EcoR I and Pst I.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/07/Tn-2013-K592016_in_J61002.jpg\" width=\"450px\" /></center></html>}}<html> </html>",
+	"content" : "<html>today i saw the plates of the cloning that i did yesterday and tha control plate was a mess... there were too many colonies!!i will do some inocula this evening..i try to redo a new ligation adding the SAP after the digestion of EFE in pUC57. Meanwhile i will start a new overnight digestion trying to insert EFE in the PsB1C3 backbone of the part s04617.</html>",
-	"tags" : "K592016"
+	"tags" : "s04617-EFE"
 }