From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-08-15",
| + | "date" : "2013-08-15", |
| - | "author" : "emil",
| + | "author" : "viola-fabio", |
| - | "title" : "Emil's (re)begins",
| + | "title" : "Verifying the triple ligation", |
| - | "content" : "<html> I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the results:</html> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href= 'https://2013.igem.org/Team:UNITN-Trento/ Protocols# Promega- PCR- Gel'> purification protocol</a> with the following results:</html> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html> <center> <table> <td> <th>Sample</th> <th>Quantities</th> </td> <td> <tr>GFP 1</tr> <tr>70.5 ng/6micro;l</tr> </td> <td> <tr>GFP 2</tr> <tr>52 ng/6micro;l</tr> </td> <td> <tr>PXyl 1</tr> <tr>110.4 ng/6micro;l</tr> </td> <td> <tr>Pxyl 2</tr> <tr>77.7 ng/6micro;l</tr> </td> <td> <tr>Pxyl 3</tr> <tr>137 ng/6micro;l</tr> </td> </table> </center> </html>}} <html>After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the <a href='https: //2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>", | + | "content" : "<html> In order to verify the triple ligation performed <a href= \"https://2013.igem.org/ wiki/index.php?title=Team:UNITN-Trento/ Notebook# tn- post- 2013-08-14-viola-thomas\"> the day before yesterday</a> Fabio did some inocula from the colonies of the 1: 2 condition plate. </html>", |
| - | "tags" : "PXyl-GFP"
| + | "tags" : "pFixK-cI-pLambda-EFE" |
| | } | | } |
Latest revision as of 09:13, 3 October 2013
{
"date" : "2013-08-15",
"author" : "viola-fabio",
"title" : "Verifying the triple ligation",
"content" : "In order to verify the triple ligation performed the day before yesterday Fabio did some inocula from the colonies of the 1:2 condition plate. ",
"tags" : "pFixK-cI-pLambda-EFE"
}
"
