Team:UNITN-Trento/Notebook/Labposts/08/41

From 2013.igem.org

(Difference between revisions)
(Created page with "{"date" : "2013-08-18","author" : "thomas","title" : "<html>Preparing sequencing samples of AraC-pBAD EFE</html>","content" : "<html>This morning I got to prepares samples of Ara...")
 
(One intermediate revision not shown)
Line 1: Line 1:
-
{"date" : "2013-08-18","author" : "thomas","title" : "<html>Preparing sequencing samples of AraC-pBAD EFE</html>","content" : "<html>This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers.  So knowing the preps concentrations, I prepared the mix as follow:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><table><tr><th></th><th>Volume added to sample 1:1 (&micro;l)</th><th>Volume added to sample 1:2 (&micro;l)</th></tr><tr><th>Template DNA</th><td>5.5</td><td>6.5</td></tr><tr><th>Primer</th><td>2</td><td>2</td></tr><tr><th>Water</th><td>up to 15</td><td>up to 15</td></tr></table></html>}}<html> The samples were then putted in a falcon and send with a courier. </html>","tags" : "aracpbad-efe"}
+
{
 +
"date" : "2013-08-15",
 +
"author" : "caterina",
 +
"title" : "fail with honor",
 +
"content" : "<html>Starting from the o/n cultures prepared yesterday, I performed a miniprep purification and then, I proceeded then performing a screening digestion followed by an electrophoresis analysis (Kapa universal ladder was adopted).</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/8/87/Tn-2013_Fail_J_016%2Bs17.jpg\" /></center></html>}}<html> As you can see, I failed the ligation of s04617 in J61002 with k592016. </html>",
 +
"tags" : "light receptor"
 +
}

Latest revision as of 09:14, 3 October 2013

{ "date" : "2013-08-15", "author" : "caterina", "title" : "fail with honor", "content" : "Starting from the o/n cultures prepared yesterday, I performed a miniprep purification and then, I proceeded then performing a screening digestion followed by an electrophoresis analysis (Kapa universal ladder was adopted).

Gel Image
As you can see, I failed the ligation of s04617 in J61002 with k592016. ", "tags" : "light receptor" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/08/41"