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Team:UNITN-Trento/Notebook/Labposts/08/44
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(Created page with "{ "date" : "2013-08-16", "author" : "emil", "title" : "<html> To be continued</html>", "content" : "<html> I added 1 µl of DPN1 and SAP respectively to the insert and t...")
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(Created page with "{ "date" : "2013-08-16", "author" : "emil", "title" : "<html> To be continued</html>", "content" : "<html> I added 1 µl of DPN1 and SAP respectively to the insert and t...")
Newer edit →
Revision as of 15:46, 28 September 2013
{ "date" : "2013-08-16", "author" : "emil", "title" : " To be continued", "content" : " I added 1 µl of DPN1 and SAP respectively to the insert and to the backbone, after 2 hours I inactivated the enzymes by heating for 20 minutes at 80 °. Afterwards I purified the samples following the purification protocol .Then I performed the ligation of the 2 parts following the ligation protocol . Finally I transformed the entire ligation in E. coli.", "tags" : "tag1-tag2-..." }
