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Team:UNITN-Trento/Notebook/Labposts/08/44
From 2013.igem.org
(Difference between revisions)
(Created page with "{ "date" : "2013-08-16", "author" : "emil", "title" : "<html> To be continued</html>", "content" : "<html> I added 1 µl of DPN1 and SAP respectively to the insert and t...") |
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"title" : "<html> To be continued</html>", | "title" : "<html> To be continued</html>", | ||
"content" : "<html> I added 1 µl of DPN1 and SAP respectively to the insert and to the backbone, after 2 hours I inactivated the enzymes by heating for 20 minutes at 80 °. Afterwards I purified the samples following the <a href=\"http://ita.promega.com/~/media/Files/Resources/ProtCards/Wizard%20SV%20Gel%20and%20PCR%20Clean-Up%20System%20Quick%20Protocol.pdf\"> purification protocol </a>.Then I performed the ligation of the 2 parts following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\"> ligation protocol </a>. Finally I transformed the entire ligation in E. coli.</html>", | "content" : "<html> I added 1 µl of DPN1 and SAP respectively to the insert and to the backbone, after 2 hours I inactivated the enzymes by heating for 20 minutes at 80 °. Afterwards I purified the samples following the <a href=\"http://ita.promega.com/~/media/Files/Resources/ProtCards/Wizard%20SV%20Gel%20and%20PCR%20Clean-Up%20System%20Quick%20Protocol.pdf\"> purification protocol </a>.Then I performed the ligation of the 2 parts following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\"> ligation protocol </a>. Finally I transformed the entire ligation in E. coli.</html>", | ||
| - | "tags" : " | + | "tags" : "pXyl-GFP" |
} | } | ||
Latest revision as of 15:49, 28 September 2013
{ "date" : "2013-08-16", "author" : "emil", "title" : " To be continued", "content" : " I added 1 µl of DPN1 and SAP respectively to the insert and to the backbone, after 2 hours I inactivated the enzymes by heating for 20 minutes at 80 °. Afterwards I purified the samples following the purification protocol .Then I performed the ligation of the 2 parts following the ligation protocol . Finally I transformed the entire ligation in E. coli.", "tags" : "pXyl-GFP" }
