Team:UNITN-Trento/Notebook/Labposts/09/05

From 2013.igem.org

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(Created page with "{ "date":"2013-09-15", "author":"viola", "title":"<html>Bacillus, you stinks!!!</html>", "content":"<html>After the growth curve and the measures at the GC i felt an horrible sme...")
 
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{
{
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"date":"2013-09-15",
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"date" : "2013-09-10",
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"author":"viola",
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"author" : "fabio",
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"title":"<html>Bacillus, you stinks!!!</html>",
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"title" : " <html> EFE produced upon blue light illumination!!that’s totally awesome!!</html> ",
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"content":"<html>After the growth curve and the measures at the GC i felt an horrible smell... sulfur and methane?? So i tried  the lead acetate strip test to see the presence of sulfur compounds... it's true!!! we don't know why, maybe if efe is expressed and produces ethylene it becomes rapidly a mercapto compund! </html>",
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"content" : " <html> in these last days we got our two final complete circuits!! Both my cloning and pedro’s succeeded so now we have a circuit that produces amilCP+EFE with blue light, and a circuit that produces amilGFP+EFE in the dark. We took some gas cromatografic measurements afterr an evaluated induction time with our LED to see if the devices actually produces ethylene. We could only see a really huge peak) ethylene in the sample under blue light with the final circuit with inverter. So the circuit with inverter seems to work, at least usually. We could not observe ethylene with the circuit that I cloned though.</html> ",
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"tags":"EFE-BACILLUS"
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"tags" : "blue_light"
}
}

Latest revision as of 09:48, 3 October 2013

{ "date" : "2013-09-10", "author" : "fabio", "title" : " EFE produced upon blue light illumination!!that’s totally awesome!! ", "content" : " in these last days we got our two final complete circuits!! Both my cloning and pedro’s succeeded so now we have a circuit that produces amilCP+EFE with blue light, and a circuit that produces amilGFP+EFE in the dark. We took some gas cromatografic measurements afterr an evaluated induction time with our LED to see if the devices actually produces ethylene. We could only see a really huge peak) ethylene in the sample under blue light with the final circuit with inverter. So the circuit with inverter seems to work, at least usually. We could not observe ethylene with the circuit that I cloned though. ", "tags" : "blue_light" }