Team:UNITN-Trento/Notebook/Labposts/09/06

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:48, 3 October 2013

{ "date" : "2013-09-10", "author" : "gabriele", "title" : "A long day", "content" : "I prepared competent NEB10β cells with the usual protocol. I miniprepped mutB inocula and prepared inocula of pLac+SAMsynthetase and Wintergreen+pLac+SAMsynthetase. I plated the new competent cells on CM to detect any contamination. I performed a PCR to extract EFE from pucEFE3 (buffer HF).I also purified Viola's EFE PCRs and digested EFE (PCR insert, XbaI and PstI) and mutB (SpeI and PstI) overnight.", "tags" : "EFE-BlueLight" }

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- {
+{
-    "date" : "2013-09-12",
+	"date" : "2013-09-10",
-    "author" : "thomas-michele",
+	"author" : "gabriele",
-    "title" : "To ripen or not to ripen? This is the problem!?",
+	"title" : "A long day",
-    "content" : "This is the last week of experiments on the fruit maturation! We will miss for sure the fantastic routine of preparing all the ingredients for an optimal maturation but after all that tries we were finishing all the stocks of the Sigma-Aldrich LB! Hoping that the results of these last experiments will be good!! Goodbay dear fruits, we are sure that from now you will be able to answer the famous question in an easier way!",
+	"content" : "<html>I prepared competent NEB10&beta; cells with the usual protocol. I miniprepped mutB inocula and prepared inocula of pLac+SAMsynthetase and Wintergreen+pLac+SAMsynthetase. I plated the new competent cells on CM to detect any contamination. I performed a PCR to extract EFE from pucEFE3 (buffer HF).I also purified Viola's EFE PCRs and digested EFE (PCR insert, XbaI and PstI) and mutB (SpeI and PstI) overnight.</html>",
-    "tags" : "fruit"
+	"tags" : "EFE-BlueLight"
 }