Team:UNITN-Trento/Notebook/Labposts/09/07

From 2013.igem.org

(Difference between revisions)
(Created page with "{ "date" : "2013-09-09", "author" : "gabriele", "title" : "Amplifications", "content" : "<html>I amplified the confirmed constructs (pLAC+SAMsynthetase and the other one with...")
 
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{
{
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"date" : "2013-09-09",
+
"date" : "2013-09-11",
"author" : "gabriele",
"author" : "gabriele",
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"title" : "Amplifications",
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"title" : "Cloning",
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"content" : "<html>I amplified the confirmed constructs (pLAC+SAMsynthetase and the other one with Wintergreen) in NEB10&beta;</html>",
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"content" : "<html>I treated the o/n digestion of mutB with SAP and of EFE with DpnI. I miniprepped yesterday inocula with bad results (I used a new kit) and screened EFE PCR, which was positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|PCR Screening|<html><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-20130911_EFEpcr.jpg\" /></html>}}<html>Then I ligated the purified O/N digestions: Ctrl, 1:1 and 1:2. I transformed the ligation in NEB10&beta; and plated them.</html>",
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"tags" : "MeSA"
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"tags" : "EFE-BlueLight"
}
}

Latest revision as of 09:48, 3 October 2013

{ "date" : "2013-09-11", "author" : "gabriele", "title" : "Cloning", "content" : "I treated the o/n digestion of mutB with SAP and of EFE with DpnI. I miniprepped yesterday inocula with bad results (I used a new kit) and screened EFE PCR, which was positive!

Then I ligated the purified O/N digestions: Ctrl, 1:1 and 1:2. I transformed the ligation in NEB10β and plated them.", "tags" : "EFE-BlueLight" }