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Team:UNITN-Trento/Parts
From 2013.igem.org
(Difference between revisions)
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In this beautiful page you can find <i>the fruits</i> of our summer’s efforts, otherwise you can just go to the <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&group=UNITN-Trento" target="_blank">official page</a>. | In this beautiful page you can find <i>the fruits</i> of our summer’s efforts, otherwise you can just go to the <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&group=UNITN-Trento" target="_blank">official page</a>. | ||
<p> | <p> | ||
| - | The number of bananas | + | The number of bananas indicates: |
</p> | </p> | ||
<ul> | <ul> | ||
| Line 19: | Line 19: | ||
<li><img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed. </li> | <li><img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" />: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed. </li> | ||
| - | <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part was characterized but doesn't work always as | + | <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part was characterized but doesn't work always as expected.</li> |
<li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part works as expected and has been characterized.</li> | <li><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" />: the part works as expected and has been characterized.</li> | ||
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<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. | + | PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. This is another part from the '<i>Eau de coli</i>' MIT iGEM 2006 project that we re-submitted (<i>and resurrected</i>) in pSB1C3. |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 203: | Line 203: | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | The wintergreen device: PchBA under a | + | The wintergreen device: PchBA under a Lac promoter followed by BSMT1 under the araCpBAD promoter. |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 238: | Line 238: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light circuit to produce amilGFP</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | A device to characterize our improved part BBa_K1065305 controlled by pLac promoter. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 255: | Line 257: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td> |
| - | <td class="name"> | + | <td class="name">BBa_K952003 improved</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 272: | Line 276: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065309">BBa_K1065309</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light ethylene producing device</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 289: | Line 294: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> |
| - | <td class="name"> | + | <td class="name">Complete blue-light sensing circuit with an inverter</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 306: | Line 313: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/1/ | + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td> |
| - | <td class="name"> | + | <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 323: | Line 331: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td> |
| - | <td class="name"> | + | <td class="name">J23100+Yf1+FixJ</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 342: | Line 348: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065307">BBa_K1065307</a></td> |
| - | <td class="name"> | + | <td class="name">J23100+Yf1+FixJ (with terminators)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 361: | Line 365: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | |
| - | + | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065308">BBa_K1065308</a></td> |
| - | <td class="name"> | + | <td class="name">BBa_K1065307 with inverter</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 379: | Line 382: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td> |
| - | <td class="name"> | + | <td class="name">Yf1+FixJ+pFixK2</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | Yf1 and FixJ | + | The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 398: | Line 399: | ||
<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" /> | |
| - | + | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065300">BBa_K1065300</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light circuit to produce amilGFP</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | It does not work beacuse it lacks the RBS before the amilGFP gene. | |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 430: | Line 430: | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | + | A <i>B. subtilis</i> xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 447: | Line 447: | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | EFE in pSpac vector, specific for B. subtilis. | + | EFE in pSpac vector, specific for <i>B. subtilis</i>. |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 464: | Line 464: | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | EFE in pXyl vector, specific for B. subtilis. | + | EFE in pXyl vector, specific for <i>B. subtilis</i>. |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 475: | Line 475: | ||
<div class="container"> | <div class="container"> | ||
| - | + | <div class="sheet-2"> | |
| - | + | <a href="https://2013.igem.org/Team:UNITN-Trento/Modeling"> | |
| - | + | <img class="tn-arr-prev" src="https://static.igem.org/mediawiki/2013/b/be/Tn-2013-arr-Parts_prev.png" /> | |
| - | + | </a> | |
| - | + | <a href="javascript:toTop('#tn-main-wrap-wrap');"> | |
| - | + | <img src="https://static.igem.org/mediawiki/2013/6/6e/Tn-2013-arr-AAA_TOP.png" /> | |
| - | + | </a> | |
| - | + | <a href="https://2013.igem.org/Team:UNITN-Trento/Project/Datapage"> | |
| - | + | <img class="tn-arr-next" src="https://static.igem.org/mediawiki/2013/f/fb/Tn-2013-arr-Parts_next.png" /> | |
| - | + | </a> | |
| - | + | </div> | |
</div> | </div> | ||
<!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/e/ef/Tn-2013-headerbg-Sfondogr2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/5/52/Tn-2013-headerbg-Sfondogr2_OR.jpg</html>}} | <!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/e/ef/Tn-2013-headerbg-Sfondogr2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/5/52/Tn-2013-headerbg-Sfondogr2_OR.jpg</html>}} | ||
Latest revision as of 15:48, 4 October 2013
In this beautiful page you can find the fruits of our summer’s efforts, otherwise you can just go to the official page.
The number of bananas indicates:
: This is an intermediate part and it has not been characterized.
: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed. - : the part was characterized but doesn't work always as expected.
- : the part works as expected and has been characterized.
♫ ♪ “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” ♪ ♫
[cit. The Banana Splits – The Tra La La Song]
|
|
BBa_K1065000 | EFE |
| Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. | ||
|
|
BBa_K1065002 | EFE with double-terminator |
| EFE followed by the double terminator BBa_B0015. | ||
|
|
BBa_K1065001 | EFE arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
|
|
BBa_K1065100 | BSMT1 |
| This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3. | ||
|
|
BBa_K1065103 | PchBA |
| PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. This is another part from the 'Eau de coli' MIT iGEM 2006 project that we re-submitted (and resurrected) in pSB1C3. | ||
|
|
BBa_K1065104 | SAM synthetase |
| This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules. | ||
|
|
BBa_K1065101 | BSMT1 arabinose-regulated |
| A methyltransferase (BSMT1) regulated by the araCpBAD promoter. | ||
|
|
BBa_K1065105 | SAM synthetase under pLac promoter |
| This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter. | ||
|
|
BBa_K1065102 | PchBA under pLac and BSMT1 arabinose-regulated |
| The wintergreen device: PchBA under a Lac promoter followed by BSMT1 under the araCpBAD promoter. | ||
|
|
BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production. | ||
|
|
BBa_K1065302 | Blue light circuit to produce amilGFP |
| A device to characterize our improved part BBa_K1065305 controlled by pLac promoter. | ||
|
|
BBa_K1065305 | BBa_K952003 improved |
| No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works. | ||
|
|
BBa_K1065309 | Blue light ethylene producing device |
| Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | ||
|
|
BBa_K1065310 | Complete blue-light sensing circuit with an inverter |
| Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | ||
|
|
BBa_K1065311 | Complete blue-light sensing device with inverter, amilCP and EFE |
| Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | ||
|
|
BBa_K1065306 | J23100+Yf1+FixJ |
| Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). | ||
|
|
BBa_K1065307 | J23100+Yf1+FixJ (with terminators) |
| The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us. | ||
|
|
BBa_K1065308 | BBa_K1065307 with inverter |
| This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us. | ||
|
|
BBa_K1065303 | Yf1+FixJ+pFixK2 |
| The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. | ||
|
|
BBa_K1065300 | Blue light circuit to produce amilGFP |
| It does not work beacuse it lacks the RBS before the amilGFP gene. | ||
|
|
BBa_K1065201 | pXyl+GFP |
| A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | ||
|
|
BBa_K1065203 | Efe+Bba_B0015 in pSpac. |
| EFE in pSpac vector, specific for B. subtilis. | ||
|
|
BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
