Team:UNITN-Trento/Project/Bacillus

From 2013.igem.org

(Difference between revisions)
(Added nav & tour)
Line 14: Line 14:
     <div class="sheet">
     <div class="sheet">
         <div>
         <div>
-
             <h1>
+
             <span class="tn-title">
Bacillus Subtilis
Bacillus Subtilis
-
</h1>
+
</span>
 +
When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:
 +
<ol>
 +
<li>
 +
<i>Bacillus subtilis</i> sporulates and it can be stored in a inactive state;
 +
</li>
 +
<li>
 +
<i>Bacillus subtilis</i> is not pathogenic;
 +
</li>
 +
<li>
 +
we have always worked only with <i>E. coli</i> and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!
 +
</li>
 +
</ol>
 +
 +
<i>Bacillus subtilis</i> would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a <i>B. fruity</i> home edition that exploits this principle. <br/>
 +
To achieve this goal we started working with EFE, a ethylene forming enzyme from <i>Pseudomas Syringae</i> pv. phaseolicola (<a href="http://parts.igem.org/Part:BBa_K1065002">BBa_K1065002</a>), which were inserted into pSBBs0K-Pspac (IPGT inducible) and  pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for <i>B. subtilis</i> by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
 +
<img src="https://static.igem.org/mediawiki/2013/8/85/Tn-2013-project_ethylene-BBa_K1065001.jpg">
 +
         </div>
         </div>
     </div>
     </div>

Revision as of 08:34, 30 September 2013

Bacillus Subtilis When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:
  1. Bacillus subtilis sporulates and it can be stored in a inactive state;
  2. Bacillus subtilis is not pathogenic;
  3. we have always worked only with E. coli and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!
Bacillus subtilis would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a B. fruity home edition that exploits this principle.
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
Edit this page | Main Page

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Project/Bacillus"