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Team:UNITN-Trento/Project/Bacillus
From 2013.igem.org
Bacillus Subtilis
When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
- Bacillus subtilis sporulates and it can be stored in a inactive state;
- Bacillus subtilis is not pathogenic;
- we have always worked only with E. coli and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
