Team:UNITN-Trento/Project/Home Edition

From 2013.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
-
<!--NEW PAGE-->
 
{{:Team:UNITN-Trento/Templates/Default|<html><!--start content-->
{{:Team:UNITN-Trento/Templates/Default|<html><!--start content-->
<!--CSS-->
<!--CSS-->
-
<link rel="stylesheet" href="https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/CSS/Project_pages&action=raw&ctype=text/css" type="text/css" />
+
<link rel="stylesheet" href="https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/CSS/Project_pages&amp;action=raw&amp;ctype=text/css" type="text/css">
-
<link rel="stylesheet" href="https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/CSS/Project/Vending_Machine&action=raw&ctype=text/css" type="text/css" />
+
<link rel="stylesheet" href="https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/CSS/Project/Vending_Machine&amp;action=raw&amp;ctype=text/css" type="text/css">
-
 
+
<!--JS-->
<!--JS-->
-
<script type="text/javascript" src="https://2013.igem.org/Team:UNITN-Trento/JS/Project/Vending_Machine?action=raw&ctype=text/javascript"></script>
+
<script type="text/javascript" src="https://2013.igem.org/Team:UNITN-Trento/JS/Project/Vending_Machine?action=raw&amp;ctype=text/javascript"></script>
<!--PAGE-->
<!--PAGE-->
<div class="container">
<div class="container">
<div class="sheet">
<div class="sheet">
-
<center><h1>B. fruity - Home Edition</h1>
+
<span class="tn-title"><i>B. fruity</i> - Home Edition<span class="tn-subscript" style="padding-left:0!important;">Our household theoretical product.</span></span>
-
<span class="subscript">Our household theoretical product.</span>
+
<span class="tn-subtitle">Why?</span>
-
</center>
+
-
+
<p>
<p>
-
<span class="subtitle">Why?</span>
+
Home Edition is conceived thinking about what families and regular fruit consumer need: a further way to control fruit ripening <b>at home</b>. There are plenty of occasions in which getting ripe fruit in time is needed, and just as much in which we want our fruit to stay green as long as possible. Home Edition is a reliable, easy to use, paper bag that can speed up or slow down ripening of the fruit that you can find in any family’s kitchen!!
-
Home Edition is conceived thinking about what families and regular fruit consumer need: a further way to control fruit ripening <b>at home</b>. There are plenty of occasions in which getting ripe fruit in time is needed, and just as much in which we want our fruit to stay green as long as possible.
+
-
 
+
-
Home Edition is a reliable, easy to use, paper bag that can speed up or slow down ripening of the fruit that you can find in any family’s kitchen!!  
+
</p>
</p>
-
<img src="https://static.igem.org/mediawiki/2013/a/a5/Tn-2013_Home_edition_1.jpg" style="width:80%;box-shadow:2px 2px 4px #323232; border:2px solid white; border-radius:0!important;"/><br/>
 
-
<p>
 
-
We chose paper as the main material since fruit perspiration would be restricted if the bag was in plastic. The sack is designed to have a little container on the inside called <b>the pocket</b>: it contains two semi-permeable bags with engineered B. subtilis that allow the molecules (in one case ethylene and in the other MeSA) to exit and act on fruit, but at the same time avoid Bacillus to get outside. We went for Bacillus subtilis as our chassis because it forms endospores, so it can be stored in a dormant and safe state. Taking in consideration the marketability of the idea, the capacity to turn into spores seems to be convenient and suitable for Home Edition.
 
-
<br/>Therefore ethylene/ MeSA production is avoided until fresh broth is added.<br/>
+
<img src="https://static.igem.org/mediawiki/2013/a/a5/Tn-2013_Home_edition_1.jpg" />
-
For this device we took inspiration from groeningen 2012 igem team’s “sticker”, a fascinating device that they designed and actually built in order to detect and report volatiles in spoiling meat.
+
<p>
 +
We chose paper as the main material since fruit perspiration would be restricted if the bag was in plastic. The sack is designed to have a little container on the inside called <b>the pocket</b>: it contains two semi-permeable pods with engineered <i>B. subtilis</i> that allow the molecules (in one case ethylene and in the other MeSA) to exit and act on fruit, but at the same time avoid Bacillus to get outside. We went for <i>Bacillus subtilis</i> as our chassis because it forms endospores, so it can be stored in a dormant and safe state. Taking in consideration the marketability of the idea, the capacity to turn into spores seems to be convenient and suitable for Home Edition.<br>
 +
</p>
</p>
 +
<span class="tn-subtitle">The pocket</span>
<p>
<p>
-
<span class="subtitle">The pocket</span>
+
The paper bag is equipped with two different plastic pods that can be placed in the pocket allowing whether ethylene production or MeSA production: both are made of two compartments, one with bacillus spores, and the other (breakable) with nutrient and the respective inducer.  
-
The paper bag is equipped with two different plastic sachets that can be placed in the pocket allowing whether ethylene production or MeSA production: both are made of two compartments, one with bacillus spores, and the other (breakable) with nutrient and the respective inducer. After breaking the partition wall between the two chambers, the nutrition goes to the spores reactivating them; the inducer present in the broth enables the culture to produce the molecule demanded, that will go out through this special semi-permeable plastic.
+
The pod is built with a plastic material that allows ethylene/MeSA to escape in the paper bag, but entraps the bacteria inside the pod itself.
 +
For this device we took inspiration from <a href="https://2012.igem.org/Team:Groningen/Sticker">groningen 2012 igem team’s “sticker”</a>, a fascinating device that they designed and actually built in order to detect and report volatiles in spoiling meat.
 +
After breaking the partition wall between the two chambers, the nutrition goes to the spores reactivating them; the inducer enables the bacteria to produce the molecule demanded, that will go out through this special semi-permeable plastic.  
 +
Therefore ethylene/ MeSA production is avoided until nutrients and inducer are added.
 +
The two different sachets are coloured differently, for an easy-to-use application, one is blue-colored (MeSA) and the other is orange (ethylene).
 +
                </p>
-
Besides, two colorants are inserted in the broth, so the compartment with the inducer for MeSA synthesis will appear blue, instead the one with the inducer needed for EFE transcription will look orange.
 
-
<br/></p>
+
<span class="tn-effect">Now you have to choose which color you want to pop!!</span>
-
<center><h3>
+
 
-
Now you have to choose which color you want to pop!!
+
<p>
-
</h3></center>
+
Once you break the desired compartment, you just put the pod inside the pocket then zip the bag and here we go!!
 +
</p>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/0/07/Tn-2013_Home_edition_2.jpg" />
 +
</div>
-
<p>
+
<div class="sheet-2">
-
+
    <a href="https://2013.igem.org/Team:UNITN-Trento/Project/Vending%20Machine">
-
Once you break the desired broth compartment, you just put the sachet inside the pocket then close the gap on the top and here we go!!</p>
+
        <img class="tn-arr-prev" src="https://static.igem.org/mediawiki/2013/d/df/Tn-2013-arr-HomeEdition_prev.png" />
-
+
    </a>
-
<img src="https://static.igem.org/mediawiki/2013/0/07/Tn-2013_Home_edition_2.jpg" style="width:80%;box-shadow:2px 2px 4px #323232; border:2px solid white;border-radius:0!important;"/>
+
    <a href="javascript:toTop('#tn-main-wrap-wrap');">
 +
        <img src="https://static.igem.org/mediawiki/2013/6/6e/Tn-2013-arr-AAA_TOP.png" />
 +
    </a>
 +
    <a href="https://2013.igem.org/Team:UNITN-Trento/Extra/Judging">
 +
        <img class="tn-arr-next" src="https://static.igem.org/mediawiki/2013/e/e4/Tn-2013-arr-HomeEdition_next.png" />
 +
    </a>
</div>
</div>
</div>
</div>
<!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/d/df/Tn-2013-headerbg-Sfondogr-2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/b/b2/Tn-2013-headerbg-Sfondogr.jpg</html>}}
<!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/d/df/Tn-2013-headerbg-Sfondogr-2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/b/b2/Tn-2013-headerbg-Sfondogr.jpg</html>}}

Latest revision as of 17:54, 4 October 2013

B. fruity - Home EditionOur household theoretical product. Why?

Home Edition is conceived thinking about what families and regular fruit consumer need: a further way to control fruit ripening at home. There are plenty of occasions in which getting ripe fruit in time is needed, and just as much in which we want our fruit to stay green as long as possible. Home Edition is a reliable, easy to use, paper bag that can speed up or slow down ripening of the fruit that you can find in any family’s kitchen!!

We chose paper as the main material since fruit perspiration would be restricted if the bag was in plastic. The sack is designed to have a little container on the inside called the pocket: it contains two semi-permeable pods with engineered B. subtilis that allow the molecules (in one case ethylene and in the other MeSA) to exit and act on fruit, but at the same time avoid Bacillus to get outside. We went for Bacillus subtilis as our chassis because it forms endospores, so it can be stored in a dormant and safe state. Taking in consideration the marketability of the idea, the capacity to turn into spores seems to be convenient and suitable for Home Edition.

The pocket

The paper bag is equipped with two different plastic pods that can be placed in the pocket allowing whether ethylene production or MeSA production: both are made of two compartments, one with bacillus spores, and the other (breakable) with nutrient and the respective inducer. The pod is built with a plastic material that allows ethylene/MeSA to escape in the paper bag, but entraps the bacteria inside the pod itself. For this device we took inspiration from groningen 2012 igem team’s “sticker”, a fascinating device that they designed and actually built in order to detect and report volatiles in spoiling meat. After breaking the partition wall between the two chambers, the nutrition goes to the spores reactivating them; the inducer enables the bacteria to produce the molecule demanded, that will go out through this special semi-permeable plastic. Therefore ethylene/ MeSA production is avoided until nutrients and inducer are added. The two different sachets are coloured differently, for an easy-to-use application, one is blue-colored (MeSA) and the other is orange (ethylene).

Now you have to choose which color you want to pop!!

Once you break the desired compartment, you just put the pod inside the pocket then zip the bag and here we go!!