Team:UNITN-Trento/Protocols

From 2013.igem.org

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To test if our sistem was able to force or to slow down the fruit ripening, we designed an ermetically closed jam jar with a connector.
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To test if our system was able to accelerate or to slow down fruit ripening, we designed an ermetically closed jam jar with a rubber hose connector. These jars contained our test-fruit and each one was connected to a flask. The flasks contained 300 ml of induced (or not) culture when his O.D.600 reached 0.8. The flasks contained also a stirrer. The cultures were maintained at 37 &deg;C using a laboratory heating plate connected to a digital thermometer immersed in the culture.<br/>
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This jars contained our test-fruit and each one was connected through a rubber hose to a flask.
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For six days, every morning the culture in the flasks was substituted with a new induced (or not) colture .<br/>
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The flasks contained 300 ml of culture induced (or not) when his O.D.600 reached 0.8.
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Furthermore, canonical jam jars (i.e.: with no connector) were adopted to contain the negative control fruit samples.<br/>
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The flask contained also a stirrer.
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The culture was mantained at 37 &deg;C using a laboratory heating plate connected to a digital thermometer immersed in the culture.<br/>
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Every day the flasks were exchanged with a new induced (or not) colture for six days.<br/>
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Moreover canonical jam jars were adopted to contain the negative control samples.<br/>
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Revision as of 09:36, 7 August 2013


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