Team:UNITN-Trento/Safety

From 2013.igem.org

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           <h1>Safety</h1>
           <h1>Safety</h1>
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:D SAFETY PAGE :D
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<p>
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When we decide to engineer a biological system able to produce ethylene and methyl-salycilate, we looked at all the existing natural pathways. For ethylene we firstly checked the plants producing pathway.
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</p>
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<img src="https://static.igem.org/mediawiki/2013/b/bb/Tn-2013-project_ethylene-Plants_path.jpg"/>
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<p>
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An unwanted byproduct is produced in the last step of ethylene synthesis: cyanide, an highly toxic compound that inhibits the cychrome C oxydase enzyme. Plants however have a complex detoxyfication mechanism. That's why we don't die when we eat a fruit!</p>
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<img src="https://static.igem.org/mediawiki/2013/5/5a/Tn-2013-bruno_face_2.jpg" />
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<p>
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We immediately though this detoxification path was too complicate to be insered into a microrganism. This due for example that plants enzymes often have diffent
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glycosilisation pattern that bacteria can not produce. A wrong glycosilation pattern can affect protein folding and activity. In order to avoid these problems, we quitted this path and focused on a more interesting
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one. <i>Pseudomonas Syrigae pv.</i>, a plant pathogen bacteria, is able to produce ethylene explointing only one enzyme. 2-Oxoglutarate Oxygenase/Decarboxylase enzyme takes 2-Oxoglutarate
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as substrate and transforms it into ethylene + water + carbon-dioxyde. Goto M. Plant and Cell Physiology (2012) 26, 141-150.
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</p>
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<img src="https://static.igem.org/mediawiki/2013/f/f8/Tn-2013-project_ethylene-Eth_path.jpg" />
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<a href="https://static.igem.org/mediawiki/2013/9/98/Tn-2013-UniTN_Trento_Safety.pdf">Safety form</a>;
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Revision as of 09:03, 24 September 2013

Safety

When we decide to engineer a biological system able to produce ethylene and methyl-salycilate, we looked at all the existing natural pathways. For ethylene we firstly checked the plants producing pathway.

An unwanted byproduct is produced in the last step of ethylene synthesis: cyanide, an highly toxic compound that inhibits the cychrome C oxydase enzyme. Plants however have a complex detoxyfication mechanism. That's why we don't die when we eat a fruit!

We immediately though this detoxification path was too complicate to be insered into a microrganism. This due for example that plants enzymes often have diffent glycosilisation pattern that bacteria can not produce. A wrong glycosilation pattern can affect protein folding and activity. In order to avoid these problems, we quitted this path and focused on a more interesting one. Pseudomonas Syrigae pv., a plant pathogen bacteria, is able to produce ethylene explointing only one enzyme. 2-Oxoglutarate Oxygenase/Decarboxylase enzyme takes 2-Oxoglutarate as substrate and transforms it into ethylene + water + carbon-dioxyde. Goto M. Plant and Cell Physiology (2012) 26, 141-150.

Safety form;