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Team:UNITN-Trento/Safety
From 2013.igem.org
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background-image: url(https://static.igem.org/mediawiki/2013/b/bf/Tn-2013_Onda-fruitripening.png)!important; | background-image: url(https://static.igem.org/mediawiki/2013/b/bf/Tn-2013_Onda-fruitripening.png)!important; | ||
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| + | .brunoide {width:50%; display:inline-block;} | ||
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When we decide to engineer a biological system able to produce ethylene and methyl-salycilate, we looked at all the existing natural pathways. For ethylene we firstly checked the plants producing pathway. | When we decide to engineer a biological system able to produce ethylene and methyl-salycilate, we looked at all the existing natural pathways. For ethylene we firstly checked the plants producing pathway. | ||
</p> | </p> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/b/bb/Tn-2013-project_ethylene-Plants_path.jpg"/> | + | <center><img class="path" src="https://static.igem.org/mediawiki/2013/b/bb/Tn-2013-project_ethylene-Plants_path.jpg"/></center> |
| - | <p> | + | <p style="display:inline-block; width:49%;vertical-align:middle;"> |
An unwanted byproduct is produced in the last step of ethylene synthesis: cyanide, an highly toxic compound that inhibits the cychrome C oxydase enzyme. Plants however have a complex detoxyfication mechanism. That's why we don't die when we eat a fruit!</p> | An unwanted byproduct is produced in the last step of ethylene synthesis: cyanide, an highly toxic compound that inhibits the cychrome C oxydase enzyme. Plants however have a complex detoxyfication mechanism. That's why we don't die when we eat a fruit!</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/5/5a/Tn-2013-bruno_face_2.jpg" /> | + | <img title="This is the exception that proves the rule. Bruno in fact is allergic to almost all fruit and would die in any case!!!" id="brunoide" src="https://static.igem.org/mediawiki/2013/5/5a/Tn-2013-bruno_face_2.jpg" /> |
<p> | <p> | ||
We immediately though this detoxification path was too complicate to be insered into a microrganism. This due for example that plants enzymes often have diffent | We immediately though this detoxification path was too complicate to be insered into a microrganism. This due for example that plants enzymes often have diffent | ||
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</p> | </p> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/f/f8/Tn-2013-project_ethylene-Eth_path.jpg" /> | + | <center><img class="path" src="https://static.igem.org/mediawiki/2013/f/f8/Tn-2013-project_ethylene-Eth_path.jpg" /></center> |
<a href="https://static.igem.org/mediawiki/2013/9/98/Tn-2013-UniTN_Trento_Safety.pdf">Safety form</a>; | <a href="https://static.igem.org/mediawiki/2013/9/98/Tn-2013-UniTN_Trento_Safety.pdf">Safety form</a>; | ||
Revision as of 09:36, 24 September 2013
Safety
When we decide to engineer a biological system able to produce ethylene and methyl-salycilate, we looked at all the existing natural pathways. For ethylene we firstly checked the plants producing pathway.
An unwanted byproduct is produced in the last step of ethylene synthesis: cyanide, an highly toxic compound that inhibits the cychrome C oxydase enzyme. Plants however have a complex detoxyfication mechanism. That's why we don't die when we eat a fruit!
We immediately though this detoxification path was too complicate to be insered into a microrganism. This due for example that plants enzymes often have diffent glycosilisation pattern that bacteria can not produce. A wrong glycosilation pattern can affect protein folding and activity. In order to avoid these problems, we quitted this path and focused on a more interesting one. Pseudomonas Syrigae pv., a plant pathogen bacteria, is able to produce ethylene explointing only one enzyme. 2-Oxoglutarate Oxygenase/Decarboxylase enzyme takes 2-Oxoglutarate as substrate and transforms it into ethylene + water + carbon-dioxyde. Goto M. Plant and Cell Physiology (2012) 26, 141-150.
