Team:USTC CHINA

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USTC_China

In Situ Transdermal Vaccine

Fresh Expression, No Purification
Standardized, Block-based Design
Easy-transportation, Low Storage External Costs
Almost No Demand For Medical Conditions And Proferssionals

Project

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
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Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will share a great percentage of
the total bacteria in our "band-aid".
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LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
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TNFα
TNFαis a chemotactic factor that recruits Langerhans Cells, antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for immigrating LC and triggering more intense immune responses.
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REPORTER
Reporter notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms . Further more killing switch is conveyed in them as a biosafety guard.
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Experimental Measurement

Molecules part
We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.
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Protein part
Before we achieve fresh expression, we use purified protein to obtain accurate data to verify our assumptions.
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Mice experiments
The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments
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