Team:USTC CHINA/Project/Background
From 2013.igem.org
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<h2>Design of Project<h2> | <h2>Design of Project<h2> | ||
- | <p align="justify">This year, our project focused on a revolutionary vaccine delivery. We chose Bacillus subtilis as chassis to establish a transdermal vaccine fresh secreting band-aid which consists of four engineering B.subtilis, each of them carries a gene circuit independently. By using an excellent transdermal peptide TD1, three of them could express a series of fusion proteins(antigen, 2 kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth type is our "reporter" , which could notify users whether the band-aid works well and when they could stick or tear off the patch. Moreover, we designed a reliable suicide system in B.subtilis for the very first time in iGEM. Innovative and incredible, we plan to create a world without needles.<p> | + | <p align="justify">This year, our project focused on a revolutionary vaccine delivery. We chose <i>Bacillus subtilis</i> as chassis to establish a transdermal vaccine fresh secreting band-aid which consists of four engineering <i>B.subtilis</i>, each of them carries a gene circuit independently. By using an excellent transdermal peptide TD1, three of them could express a series of fusion proteins(antigen, 2 kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth type is our "reporter" , which could notify users whether the band-aid works well and when they could stick or tear off the patch. Moreover, we designed a reliable suicide system in <i>B.subtilis</i> for the very first time in iGEM. Innovative and incredible, we plan to create a world without needles.<p> |
<h2>How do we realize T-vaccine<h2> | <h2>How do we realize T-vaccine<h2> | ||
<h3>1. Transdermal Peptide 1:<h3> | <h3>1. Transdermal Peptide 1:<h3> | ||
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4.Zhang, T., et al., Transmembrane Delivery and Biological Effect of Human Growth Hormone Via a Phage Displayed Peptide In Vivo and In Vitro. Journal of Pharmaceutical Sciences, 2010. 99(12): p. 4880-4891.<br/> | 4.Zhang, T., et al., Transmembrane Delivery and Biological Effect of Human Growth Hormone Via a Phage Displayed Peptide In Vivo and In Vitro. Journal of Pharmaceutical Sciences, 2010. 99(12): p. 4880-4891.<br/> | ||
5.Prausnitz, M.R. and R. Langer, Transdermal drug delivery. Nature biotechnology, 2008. 26(11): p. 1261-1268.<br/> | 5.Prausnitz, M.R. and R. Langer, Transdermal drug delivery. Nature biotechnology, 2008. 26(11): p. 1261-1268.<br/> | ||
- | 6.van Dijl, J.M., et al., Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion. Journal of Biotechnology, 2002. 98(2-3): p. 243-254.<br/> | + | 6.van Dijl, J.M., et al., Functional genomic analysis of the <i>Bacillus subtilis</i> Tat pathway for protein secretion. Journal of Biotechnology, 2002. 98(2-3): p. 243-254.<br/> |
- | 7.Ogasawara, N., Systematic function analysis of Bacillus subtilis genes. Research in Microbiology, 2000. 151(2): p. 129-134.<br/> | + | 7.Ogasawara, N., Systematic function analysis of <i>Bacillus subtilis</i> genes. Research in Microbiology, 2000. 151(2): p. 129-134.<br/> |
</p> | </p> |
Revision as of 12:26, 28 October 2013