Team:USTC CHINA/Project/Overview

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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li>
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                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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         <div id="overview-bar" class="basic-bar">
         <div id="overview-bar" class="basic-bar">
         <h1>Overview</h1>
         <h1>Overview</h1>
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         <p>In our world, billions of people are suffering from contagion. However, only a fraction of contagion can be prevented by the existing vaccines. The disadvantages of traditional vaccines limit their usage especially in developing countries. </p>
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         <p align="justify">In our world, billions of people are suffering from contagions while only parts of contagions can be effectively prevented by existing vaccines. The disadvantages of traditional vaccines, like  being produced and purified with strict requirements on temperature, have limited their application, especially in developing countries. </p>
<div align="center"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/2013ustc-china_traditional_vaccine_transport.jpg" width="400" height="300" />
<div align="center"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/2013ustc-china_traditional_vaccine_transport.jpg" width="400" height="300" />
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<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig1. hard shipping in some area </div></div>
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<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig1. Difficult shipping in remote areas </div></div>
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<p>This year, our project focused on a revolutionary vaccine delivery. We chose Bacillus subtilis as chassis to establish a band-aid secreting fresh transdermal vaccine consisting of four engineering B.subtilis, each of which carried a gene circuit independently.</p>
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<p align="justify">This year, our project focused on a revolutionary vaccine delivery. We bring a fresh Medication into the world, which contains an in situ expression system, and our product is a biological transdermal vaccine patch called T-vaccine. We chose <i>Bacillus subtilis</i> as chassis to establish the band-aid secreting fresh vaccines. The new vaccine consists of four engineering <i>B.subtilis</i>, each of which carried a gene circuit independently.</p>
<div align="center"><img src="https://static.igem.org/mediawiki/2013/archive/e/ed/20130923171924!2013igemustc_Standardization.png" width="400" height="350" />
<div align="center"><img src="https://static.igem.org/mediawiki/2013/archive/e/ed/20130923171924!2013igemustc_Standardization.png" width="400" height="350" />
<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig2. block-based design </div></div>
<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig2. block-based design </div></div>
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<p>With an excellent transdermal peptide TD1, three of the engineering B.subtilis could express a series of fusion proteins (the antigen and 2 kinds of adjuvants) which could penetrate the skin and work as traditional vaccine molecules. The fourth bacteria are our “reporter” which would notify users when the band-aid is working well and the patch can be pasted or tore off. Moreover, we designed a reliable suicide system in Bacillus subtilis for the very first time in iGEM. So it would be OK when the patches are thrown away into the environment. Cheap and convenient, we created a world without needle. </p>   
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<p align="justify">With an excellent transdermal peptide TD1, three of the engineering <i>B.subtilis</i> could express a series of fusion proteins (the antigen and two kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth bacteria are our "reporter" ,which would notify users whether the band-aid works well and when the patch can be pasted . Moreover, we designed a reliable suicide system in <i>B.subtilis</i> to ensure biosafety.</p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2013/9/91/2013ustc-china_Needles.png" width="400" height="350" />
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<div class="atfigure" align="center" style="width:400px;font-size:14px;">Fig3. No Needle </div></div>
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<p align="justify">T-vaccine can be stored from minus 20 to 60 Celsius, which grants its great advantage transportation and enables us to reach every remote corner of the world to help eliminate contagions from our world. It is also proved that transdermal vaccine is an effective method for a variety of pathogens such as: tuberculosis, anthrax, hepatitis B and so on. Additionally, we have created a world free from needles. Consider these advantages, T-vaccine is expected to set up a  promising vaccine research and a new development orientation.</p>   
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">Background</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">Design</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurtherWork">Further Work</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
</div></div></div>         
</div></div></div>         

Latest revision as of 12:24, 28 October 2013

Overview

In our world, billions of people are suffering from contagions while only parts of contagions can be effectively prevented by existing vaccines. The disadvantages of traditional vaccines, like being produced and purified with strict requirements on temperature, have limited their application, especially in developing countries.

Fig1. Difficult shipping in remote areas

This year, our project focused on a revolutionary vaccine delivery. We bring a fresh Medication into the world, which contains an in situ expression system, and our product is a biological transdermal vaccine patch called T-vaccine. We chose Bacillus subtilis as chassis to establish the band-aid secreting fresh vaccines. The new vaccine consists of four engineering B.subtilis, each of which carried a gene circuit independently.

Fig2. block-based design

With an excellent transdermal peptide TD1, three of the engineering B.subtilis could express a series of fusion proteins (the antigen and two kinds of adjuvants), which could penetrate the skin and work as traditional vaccine molecules. The fourth bacteria are our "reporter" ,which would notify users whether the band-aid works well and when the patch can be pasted . Moreover, we designed a reliable suicide system in B.subtilis to ensure biosafety.

Fig3. No Needle

T-vaccine can be stored from minus 20 to 60 Celsius, which grants its great advantage transportation and enables us to reach every remote corner of the world to help eliminate contagions from our world. It is also proved that transdermal vaccine is an effective method for a variety of pathogens such as: tuberculosis, anthrax, hepatitis B and so on. Additionally, we have created a world free from needles. Consider these advantages, T-vaccine is expected to set up a promising vaccine research and a new development orientation.