Team:USTC CHINA/zeyu

From 2013.igem.org

(Difference between revisions)
Ustczyw (Talk | contribs)
(Created page with "<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <!-- saved from url=(0036)https://2011.igem.org/Team:USTC...")
Newer edit →

Revision as of 04:38, 8 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:USTC-China - 20

 

Team:USTC-China

From 2013.igem.org

1 2 3 4 5 6 7


MODULE.png

RESULTS.png

GALLARY.png

MODELING.png



Achievement.gif

H2 generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water
when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H2 from water and sunlight using the
cells’ endogenous nitrogenases. However, nitrogenases have low turnover numbers and require large amounts of ATP. [FeFe]-
hydrogenases found in other organisms can have 1,000-fold higher turnover numbers and no specific requirement for ATP but
are very O2 sensitive. Certain filamentous cyanobacteria protect nitrogenase from O2 by sequestering the enzyme within internally
micro-oxic, differentiated cells called heterocysts. We heterologously expressed the [FeFe]-hydrogenase operon from Shewanella
oneidensis MR-1 in Anabaena sp. strain PCC 7120 using the heterocyst-specific promoter PhetN. Active [FeFe]-hydrogenase
was detected in and could be purified from aerobically grown Anabaena sp. strain PCC 7120, but only when the organism
was grown under nitrate-depleted conditions that elicited heterocyst formation. These results suggest that the heterocysts protected
the [FeFe]-hydrogenase against inactivation by O2.



Project
+ Details

Results12.jpg
Construct a novel system in which bacterial colonies will automatically divide into two parts after certain time & Assemble Riboswitches finely tuned by small molecules and Toggle Switches pushed on and off.




Modeling
+ Details

Mo0del.png
Bulid a model of our system to verify our design and help constructing new systems. & Analyse Riboswitches invovled in our systems.

Team
+ Details

Team-ustc.png
We are USTC-IGEMers from variant departments and grades and build our project together. We have a happy summer together and would like to share it~

Human Practice+ Details

Humanpra.png
In our project, we have always been focusing biosafety and issued our own Biosafety protocol. Furthermor, we found speacail groups and conduct surveys and open days to get people informed of synthetic biology.