Team:Washington/96WellPlateAssay

From 2013.igem.org

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Obtain <a href = "http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=-1&productId=3349439&distype=0&fromSearch=0">96-Well Plate</a></br>.
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Obtain <a href = "http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=-1&productId=3349439&distype=0&fromSearch=0">96-Well Plate</a>.</br>
1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br>
1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br>
2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br>
2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br>

Latest revision as of 22:27, 27 September 2013


96-Well Plate Assay
Obtain 96-Well Plate.
1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal.
2. Set the E. colight app with the appropriate conditions.
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.
4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C.
5. Follow the Fluorescent Analysis Protocol.