Team:Waterloo

From 2013.igem.org

(Difference between revisions)
Line 741: Line 741:
       name: 'Paul Reginato',
       name: 'Paul Reginato',
       url: 'https://static.igem.org/mediawiki/2013/b/bd/Waterloo_paul.JPG',
       url: 'https://static.igem.org/mediawiki/2013/b/bd/Waterloo_paul.JPG',
-
       description: "As the Assistant Director of Waterloo iGEM, I oversee the team’s operation by communicating with team members, professors, and others who contribute to our team. I lead team meetings, plan the trips to the jamborees, handle recruitment, handle finances, and ensure that deadlines are met. I also assist with tasks in the Lab and Design and Human Practices subdivisions of the team. I am a third-year student majoring in Biomedical Sciences as well as a candidate for the Psychology minor and Global Experience Certificate."
+
       description: "My name is Paul Reginato. I lead Waterloo iGEM's mathematical modelling subteam, and I  
 +
have an organizational/advisory role in the team at large. I designed this year's project, and it's
 +
been incredible to see it undertaken by the team. I am currently finishing up a BSc in biology
 +
with a joint honours in mathematics. Synthetic biology is my primary interest, along with related
 +
science/engineering fields. Outside of iGEM, my main hobbies are playing/listening to music and
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interior camping."
     };
     };
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         name: 'Rummy Chowdhury',
         name: 'Rummy Chowdhury',
         url: 'https://static.igem.org/mediawiki/2013/0/07/Waterloo_rummy.JPG',
         url: 'https://static.igem.org/mediawiki/2013/0/07/Waterloo_rummy.JPG',
-
         description: ""
+
         description: "iGEM Lab and Design Team Project Sub-leader - Honors Biomedical Science. iGEM
 +
has been one of the most rewarding experiences I’ve had. Not only has it played a major role in
 +
defining my carrier path, but it has also given me the chance to learn and expand my knowledge
 +
and meet incredible science junkies like myself."
       },
       },
     ];
     ];

Revision as of 01:36, 9 September 2013

Idea

Due to its nature as an information-encoding molecule, the use of DNA as an intercellular messaging molecule would enable more information-rich communication between populations of cells than traditional AHL-based messaging. The first demonstration of DNA messaging was published by the Endy group at Stanford University in late 2012, wherein DNA encoding instructions for expression of fluorescence and antibiotic resistance were transmitted from one bacterial population to another, carried by M13 bacteriophage particles.

Incorporation of well-established in vivo DNA modification techniques into DNA messaging will diversify and extend potential intercellular communication programs, and will enable the integration of recent developments in DNA digital logic with DNA messaging.

The goal of our project is to place on a DNA message a switch that can be flipped in receiver cells under inducible conditions, and whose state determines whether or not the DNA message is retransmitted from receiver cells to a population of secondary receiver cells. The switch consists of a promoter that can be inverted using a serine integrase, leading to transcription of different genes. It is directly inspired by the recombinase addressable data (RAD) module published by the Endy group in early 2012.

We have synthesized four such DNA switches and will soon test the ability of PhiC31 and Bxb1 serine integrases, along with the respective recombination directionality factors (RDFs), to control their states. We have also produced constructs that we will use to attempt to control the production of M13 viral particles containing a DNA message and we will test these soon. We will integrate these efforts to demonstrate our goal of incorporating digital DNA logic into DNA messaging. Through this work, we will broaden the horizons of engineered intercellular communication.

Video

Accomplishments

Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments Accomplishments

Future Aspirations

Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations Future Aspirations

Results

Results Results Results Results Results Results Results Results Results Results Results

Conclusions

Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions Conclusions

BioBricks

  • Hpdo with no gene 8
  • Bxb1 rdf
  • Bxb1 int
  • Bxb1 switch
  • BXB1 switch flipped
  • ΦC31 rdf
  • ΦC31 int
  • ΦC31 switch
  • ΦC31 switch flipped

Ottawa's Collaboration

Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa Ottawa

Notebook

Switch Modelling

x y z

Population & Infection Modelling

a b c

Phage Particle Production Modelling

a b c

Vlogs

Special Project

Intent to Invent

Laboratory

Safety

This page is still in progress

Sponsors

Administrators

Lab & Design

M13 Group

BxB1 Group

Φ C31 Group

Human Practices

Mathematical Modelling

Advisors

Graduate Student Advisors

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