"
Template:Team:SydneyUni Australia/Calendar/Events List
From 2013.igem.org
(Difference between revisions)
| Line 467: | Line 467: | ||
start: new Date(2013, 9, 23), | start: new Date(2013, 9, 23), | ||
description: '<b>Members:</b> Rob, Hugh, Andrew <br> <b>What we did: </b> Patching and lysing cells from transformation plates for PCR screen and pH-chloroacetate screens. No efficient cloning for whole pathway, only for individual parts with tetR-inducible promoter system. Set-up chloride assay for degradation of DCA by clones containing tetR-p450 and tetR-aldA-dhlB-dhlA.' | description: '<b>Members:</b> Rob, Hugh, Andrew <br> <b>What we did: </b> Patching and lysing cells from transformation plates for PCR screen and pH-chloroacetate screens. No efficient cloning for whole pathway, only for individual parts with tetR-inducible promoter system. Set-up chloride assay for degradation of DCA by clones containing tetR-p450 and tetR-aldA-dhlB-dhlA.' | ||
| + | }, | ||
| + | { | ||
| + | title: 'Chloride assay', | ||
| + | start: new Date(2013, 9, 24), | ||
| + | description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Performed chloride assay. Appears there is reasonable degradation of DCA by p450. | ||
| + | ' | ||
| + | }, | ||
| + | { | ||
| + | title: 'Acetaldehyde growth inhibition', | ||
| + | start: new Date(2013, 9, 25), | ||
| + | description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Set-up an experiment to confirm aldA expression through growth inhibition of control E. coli in LB-acetaldehyde.' | ||
| + | }, | ||
| + | { | ||
| + | title: 'Characterising new parts', | ||
| + | start: new Date(2013, 9, 26), | ||
| + | description: '<b>Members:</b> Rob, Andrew <br> <b>What we did: </b> Another LB-acetaldehyde growth experiment in triplicate with a promising tetR-aldA clone. Incubated tetR-p450 clones with DCA in triplicate to confirm result from the 24th October, after 6 hours incubation, GC was not promising. Set-up an ethene-epoxide assay to confirm p450 expression. ' | ||
} | } | ||
] | ] | ||
Revision as of 04:58, 28 October 2013
