File:HisC Positive Control.jpg

From 2013.igem.org

HisC knockout cells were transformed with the pUC19 plasmid with the extant E. coli HisC gene insert and were induced with IPTG. We allowed these cells to grow on M9 minimal media plates, so that only transformed cells would be rescued. This is a positive control for our ancestral HisC gene.

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current23:19, 27 September 2013Thumbnail for version as of 23:19, 27 September 20133,456×2,304 (1.19 MB)Tkalkus (Talk | contribs) (HisC knockout cells were transformed with the pUC19 plasmid with the extant E. coli HisC gene insert and were induced with IPTG. We allowed these cells to grow on M9 minimal media plates, so that only transformed cells would be rescued. This is a positive)

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