Team:Heidelberg/Templates/Delftibactin week15


05-08 - 11-08-13

Melting Gold Nanoparticles to Solid Gold

Step 1: Cultivation of D. acidovorans
200 ml ACM medium were inocculated with recently obtained D. acidovorans SPH-1 from glycerol stock and incubated at 30°C for 3 days.

Step 2: Precipitation of Gold Nanoparticles

  • D. acidovorans culture in ACM was divided in 50 ml aliquots and centrifuges at 3,750 rpm for 30 min.
  • Supernatant was transferred to fresh tube.
  • 300 µl gold chloride stock solution [µg/ml] was added to D. acidovorans supernatant, suspention mixed and incubated at RT for 20 min.

Step 3: Aggregation of gold nanoparticles.

Step 4: Melting gold nanoparticles to solid gold.

Alternatively, we also performed the protocol described above in a 2 ml tube.