Team:Paris Bettencourt/Notebook/Monday 1st July/Drug Screen

From 2013.igem.org

4 strains were received from Jake:

  • E. coli: BL21 (DE3) ko20 ΔcysI, Δfpr, ΔydbK
  • E. coli: NEBTurbo zmSIR Chloramphenicol
  • E. coli: NEBTurbo zmFNR Spectinomycin
  • E. coli: NEBTurbo soFD, zmSIR Chloramphenicol
Media and Glycerol stock were prepared:

Media Preparation
3 500ml bottles of LB broth and LB agar were prepared by standard methods.
12.5g/500ml powder/water for broth and 20g/500ml powder/water for agar.
Bottles were autoclaved. Additionally 1 flask of 500ml of LB broth was made in the same fashion.

Glycerol Stocks
Single colonies from agar plates were picked and used to innoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001, sD002, sD003, sD004; one set was frozen at -20ºC and the other set was frozen at -80ºC.