Team:Paris Bettencourt/Notebook/Phage Sensor/Friday 6th September.html

Phage Sensor

ASDF

Friday 6^th September

Place your twit here

Digestions: linearized pSB1C3, linearized pSB1A3, SPCR2, SPCR3, SPCR11

Reagent	Volume
Purified PCR Product	16 µl
H2OH2O	0 µl
10x FastDigest Buffer	2 µl
FastDigest Enzyme 1	1 µl
cFastDigest Enzyme 2	1 µl
Total Volume	4 µl

SPCR5, pSB1C3: XbaI/SpeI
SPCR11, pSB1A3, pSB1C3: EcoRI/PstI
SPCR13, pSB1A3, pSB1C3: EcoRI/PstI
SPCR2, pSB1A3, pSB1C3: EcoRI/PstI

1h at 37° shaking