Team:Paris Bettencourt/Notebook/Phage Sensor/Sunday 11th August.html

From 2013.igem.org

Phage Sensor

Sunday 11th August

Patches of sSP001-sSP008, sSP015-18 and Stress experiment (Plate reader)

Patches of sSP001-sSP008, sSP015-18

Stress experiment (Plate reader)
sSP001, sSP002, sSP010, sSP011, sSP012 (M13)
1:1000 bacteria in M9 Glucose
Mitomycin C concentrations: 3,3*10^-3, 1,6*10°-3, 6,6*10°-4, 3,3*10°-4
Quadruplets of bacteria (+phage), Triplets of MMC

See also pipetting scheme in folder Experiments on Dropbox

On culture of FR-433, XL-10, delta pyrF
1:1000 culture at 30°