Team:Paris Bettencourt/Notebook/Phage Sensor/Sunday 22nd September.html

From 2013.igem.org

Phage Sensor

Sunday 22nd September

PCR and Gels

PCR
M13 backbone

SPCR16,17

Reagent Volume
x1
Nuclease-free water 37.25 µl
5x Phusion HF Buffer 10 µl
10 mM dNTPs 1 µl
Forward Primer (10 uM) 0.5 µl
Reverse Primer (10 uM) 0.5 µl
Template Plasmid 0.25 µl
Phusion DNA Polymerase 0.5 µl
Total Volume 50 µl

Protocol see fotos

Gel 100V, 1%, 1,5h