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Team:TU-Delft/Notebook/2013/08/12/
From 2013.igem.org
Notebook
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12th of August
Lab work
1. pT7 which was restriction digested with S+P and X+P was heated to 80°C and 65°C respectively to deactivate the enzymes. CIP treatment was carried out at 37°C for 1 hour to remove one phosphate from the 5-prime end of the pT7.
2. Made 10X PC Buffer for Minimal media and startvation media.
3. Carried out transformations for pConst-AgrAC-pP2-GFP in XL1 Blue cells. Plated on Ampicillin plates.
