"

From 2013.igem.org

{ "date" : "2013-06-14", "author" : "caterina", "title" : "Digestion& ligation of BMST1 (BBa_J45119) and PCHA (BBa_J45319)", "content" : "I digested the pSB1C3 linearized with EcoR I and Spe I.
Next I perfomed six different ligations:
two controls with: the pSB1C3 linearized and not but without the insert
two with: pSB1C3 linearized or not and BMST1
two with: pSB1C3 linearized or not and PCHA", "tags" : "BSMT1-PchA" }