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From 2013.igem.org

{ "date" : "2013-06-18", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters (part3)!!", "content" : " from the previous inoculum ( with NEB10b cells) we could only take K823000 ( duplicate) and K143012 (triplicate). Then we performed the minipreps and quantified the yields:K823000 a = 30.7 ng/ulK823000 b = 34.2 ng/ulK143012a = 26.8 ng/ulK143012b = 30.3 ng/ulK143012c = 29.5 ng/ulAs far as NEB5a are concerned, from the inoculum we made our cells competent.In the afternoon we transformed these cells with our promoters.", "tags" : "Pveg-PliaG-PlepA" }