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From 2013.igem.org

{"date" : "2013-07-23","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP","content" : "I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates.Afterwards I transformed Neb10β with 100 ng of K823026+E0840 following the transformation protocol.","tags" : "Bacillus subtilis-pSpac+GFP"}