"

From 2013.igem.org

7/9/2013

Transformation:

[LacI] Kit plate 3. Well 1M. BBa_C0012. Resistance Chlora. I5-6-1

[pLac] Kit plate 3. Well 3H. BBa_R0010. Resistance Chlora. I5-6-2

7/10/2013

I5-6-1 →  0 colonies

I5-6-2 →  15 colonies →  picked 2 →  i5-6-3

→ I5-6-4

7/11/2013

Miniprep

I5-6-3        60.8ng/ul        [pLac]

i-5-6-4        63.8 ng/ul        [pLac]

7/12/2013

Innoculated from glycerol stock i5-3-8

7/13/2013

Miniprep

I3-5-8        134.5ng/ul        [RBS-GFP-Stop]

Digestion

<a href="#" name="670fe2ad4f411b65e5fc58b43eaedaaf31bab7cf"></a><a href="#" name="1"></a><tbody></tbody>

	I3-5-8 (1)	I5-6-4(2)
DNA	22	47
B4	5	7
BSA	5	7
XbaI	1	0
SpeI	0	1
PstI	1	1
H2O	16	7
	50	70

I5-6-5                i5-6-6

8.3 ng/ul        18.9ng/ul