LOCUS       new_mol1                7200 bp    CIRCULAR             21-AUG-2013
UNIMARK     new_mol1 annotations
            new_mol1
FEATURES             Location/Qualifiers
     source          1..7200
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    16..87
                     /gene="E. coli his operon terminator"
                     /ugene_name="misc"
     stem_loop       24..58
                     /label="terminator stem loop"
     misc_feature    28..29
                     /gene="deletion of two bases"
                     /ugene_name="misc"
     primer_bind     complement(151..170)
                     /label="VR"
     CDS             complement(233..1048)
                     /gene="kanR (rev)"
     CDS             complement(1380..2204)
                     /gene="p15A ORI (rev)"
     primer_bind     2605..2624
                     /label="VF2"
     misc_feature    complement(2681..2684)
                     /gene="end of terminator"
                     /ugene_name="misc"
     stem_loop       complement(2694..2713)
                     /label="terminator stem loop"
     misc_feature    2723..2744
                     /note="BioBrick cloning site prefix (BBa_G00000)"
     misc_feature    2745..2944
                     /label="BBa_R0010"
     misc_binding    2833..2870
                     /label="CAP"
     promoter        2881..2886
                     /label=-35
     promoter        2905..2910
                     /label=-10
     misc_binding    2910..2944
                     /label="LacI"
     misc_feature    2917..2917
                     /label="start: "
     misc_feature    complement(2947..3002)
                     /mismatches=0
                     /ugene_name="IK35"
     misc_feature    2953..2964
                     /ugene_name="BBa_B0034"
     misc_feature    2957..2960
                     /label="Conserved: "
     misc_feature    2971..4563
                     /source="Streptomyces coelicolor A3(2)"
                     /ugene_name="pccB"
     misc_feature    2971..2989
                     /mismatches=0
                     /ugene_name="IK34"
     misc_feature    4564..6354
                     /source="Streptomyces coelicolor A3(2)"
                     /ugene_name="accA2"
     misc_feature    4585..4587
                     /locus_ref="accA2"
                     /ugene_name="alternate startcodon"
     misc_feature    6325..6409
                     /mismatches=0
                     /ugene_name="IK30"
     misc_feature    complement(6334..6365)
                     /mismatches=0
                     /ugene_name="IK29"
     misc_feature    6347..6347
                     /label="putative"
                     /ugene_name="insertion"
     misc_feature    6362..6376
                     /ugene_name="BBa_B0030"
     gene            6383..7057
                     /gene="sfp gene"
     CDS             6383..7057
                     /gene="sfp gene"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA44858.1"
                     /db_xref="GI:40139"
                     /db_xref="GOA:P39135"
                     /db_xref="InterPro:IPR004568"
                     /db_xref="InterPro:IPR008278"
                     /db_xref="PDB:1QR0"
                     /db_xref="PDB:2GE0"
                     /db_xref="PDB:2GE1"
                     /db_xref="UniProtKB/Swiss-Prot:P39135"
                     /translation="MKIYGIYMDRPLSQEENERFMTFISPEKREKCRRFYHKEDAHRT
                     LLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPCIPDLPDAHFNISHSGRWVIGAFDSQ
                     PIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKDEQTDYFYHLWSMKESFIKQEGKG
                     LSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEVDPGYKMAVCAAHPDFPEDITMV
                     SYEELL"
     misc_feature    7025..7084
                     /mismatches=0
                     /ugene_name="IK32"
     misc_feature    7066..7145
                     /label="BBa_B0010"
     stem_loop       7077..7120
     misc_feature    7154..7194
                     /label="BBa_B0012"
     stem_loop       7161..7180
                     /label="T7 TE"
     polyA_signal    7181..7194
     misc_feature    7187..7187
                     /label="stop: "
     misc_feature    join(7195..7200,1..15)
                     /note="BioBrick cloning site suffix (BBa_G00001)"
ORIGIN
        1 TAGCGGCCGC TGCAGTCCGG CAAAAAAACG GGCAAGGTGT CACCACCCTG CCCTTTTTCT
       61 TTAAAACCGA AAAGATTACT TCGCGTTATG CAGGCTTCCT CGCTCACTGA CTCGCTGCGC
      121 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT CTCGAGTCCC
      181 GTCAAGTCAG CGTAATGCTC TGCCAGTGTT ACAACCAATT AACCAATTCT GATTAGAAAA
      241 ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT
      301 TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG
      361 CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT
      421 TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG
      481 GTGAGAATGG CAAAAGCTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC
      541 GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG
      601 CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC
      661 GGCGCAGGAA CACTGCCAGC GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA
      721 ATACCTGGAA TGCTGTTTTC CCGGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG
      781 TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA
      841 CCATCTCATC TGTAACATCA TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG
      901 GCGCATCGGG CTTCCCATAC AATCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC
      961 GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTCGAGC
     1021 AAGACGTTTC CCGTTGAATA TGGCTCATAA CACCCCTTGT ATTACTGTTT ATGTAAGCAG
     1081 ACAGTTTTAT TGTTCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT
     1141 GAGACACAAC GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA
     1201 CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT
     1261 GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG
     1321 GGGCGATTCA GGCCTGGTAT GAGTCAGCAA CACCTTCTTC ACGAGGCAGA CCTCAGCGCT
     1381 AGCGGAGTGT ATACTGGCTT ACTATGTTGG CACTGATGAG GGTGTCAGTG AAGTGCTTCA
     1441 TGTGGCAGGA GAAAAAAGGC TGCACCGGTG CGTCAGCAGA ATATGTGATA CAGGATATAT
     1501 TCCGCTTCCT CGCTCACTGA CTCGCTACGC TCGGTCGTTC GACTGCGGCG AGCGGAAATG
     1561 GCTTACGAAC GGGGCGGAGA TTTCCTGGAA GATGCCAGGA AGATACTTAA CAGGGAAGTG
     1621 AGAGGGCCGC GGCAAAGCCG TTTTTCCATA GGCTCCGCCC CCCTGACAAG CATCACGAAA
     1681 TCTGACGCTC AAATCAGTGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
     1741 CCCTGGCGGC TCCCTCGTGC GCTCTCCTGT TCCTGCCTTT CGGTTTACCG GTGTCATTCC
     1801 GCTGTTATGG CCGCGTTTGT CTCATTCCAC GCCTGACACT CAGTTCCGGG TAGGCAGTTC
     1861 GCTCCAAGCT GGACTGTATG CACGAACCCC CCGTTCAGTC CGACCGCTGC GCCTTATCCG
     1921 GTAACTATCG TCTTGAGTCC AACCCGGAAA GACATGCAAA AGCACCACTG GCAGCAGCCA
     1981 CTGGTAATTG ATTTAGAGGA GTTAGTCTTG AAGTCATGCG CCGGTTAAGG CTAAACTGAA
     2041 AGGACAAGTT TTGGTGACTG CGCTCCTCCA AGCCAGTTAC CTCGGTTCAA AGAGTTGGTA
     2101 GCTCAGAGAA CCTTCGAAAA ACCGCCCTGC AAGGCGGTTT TTTCGTTTTC AGAGCAAGAG
     2161 ATTACGCGCA GACCAAAACG ATCTCAAGAA GATCATCTTA TTAAGGGGTC TGACGCTCAG
     2221 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2281 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2341 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2401 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2461 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA
     2521 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2581 GCCTCCATCC AGTCTATTCC ATGGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA
     2641 CATTAACCTA TAAAAATAGG CGTATCACGA GGCAGAATTT CAGATAAAAA AAATCCTTAG
     2701 CTTTCGCTAA GGATGATTTC TGGAATTCGC GGCCGCTTCT AGAGCAATAC GCAAACCGCC
     2761 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA
     2821 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
     2881 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
     2941 CACATACTAG AGAAAGAGGA GAAATACTAG ATGTCCGAGC CGGAAGAGCA GCAGCCCGAC
     3001 ATCCACACGA CCGCGGGCAA GCTCGCGGAT CTCAGGCGCC GTATCGAGGA AGCGACGCAC
     3061 GCCGGTTCCG CACGCGCCGT CGAGAAGCAG CACGCCAAGG GCAAGCTGAC GGCTCGTGAA
     3121 CGCATCGACC TCCTCCTCGA CGAGGGTTCC TTCGTCGAGC TGGACGAGTT CGCCCGGCAC
     3181 CGCTCCACCA ACTTCGGCCT CGACGCCAAC CGCCCCTACG GCGACGGCGT CGTCACCGGC
     3241 TACGGCACCG TCGACGGCCG CCCCGTGGCC GTCTTCTCCC AGGACTTCAC CGTCTTCGGC
     3301 GGCGCGCTGG GCGAGGTCTA CGGCCAGAAG ATCGTCAAGG TGATGGACTT CGCCCTCAAG
     3361 ACCGGCTGCC CGGTCGTCGG CATCAACGAC TCCGGCGGCG CCCGCATCCA GGAGGGCGTG
     3421 GCCTCCCTCG GCGCCTACGG CGAGATCTTC CGCCGCAACA CCCACGCCTC CGGCGTGATC
     3481 CCGCAGATCA GCCTGGTCGT CGGCCCGTGT GCGGGCGGCG CGGTGTACTC CCCCGCGATC
     3541 ACCGACTTCA CGGTGATGGT GGACCAGACC AGCCACATGT TCATCACCGG TCCCGACGTC
     3601 ATCAAGACGG TCACCGGCGA GGACGTCGGC TTCGAGGAGC TGGGCGGCGC CCGCACCCAC
     3661 AACTCCACCT CGGGCGTGGC CCACCACATG GCCGGCGACG AGAAGGACGC GGTCGAGTAC
     3721 GTCAAGCAGC TCCTGTCGTA CCTGCCGTCC AACAACCTCT CCGAGCCCCC CGCCTTCCCG
     3781 GAGGAGGCGG ACCTCGCGGT CACGGACGAG GACGCCGAGC TGGACACGAT CGTCCCGGAC
     3841 TCGGCGAACC AGCCCTACGA CATGCACTCC GTCATCGAGC ACGTCCTGGA CGACGCCGAG
     3901 TTCTTCGAGA CGCAACCCCT CTTCGCGCCG AACATCCTCA CCGGCTTCGG CCGCGTGGAG
     3961 GGCCGCCCGG TCGGCATCGT CGCCAACCAG CCCATGCAGT TCGCCGGCTG CCTGGACATC
     4021 ACGGCCTCCG AGAAGGCGGC CCGCTTCGTG CGCACCTGCG ACGCCTTCAA CGTCCCCGTC
     4081 CTCACCTTCG TGGACGTCCC CGGCTTCCTG CCCGGCGTCG ACCAGGAGCA CGACGGCATC
     4141 ATCCGCCGCG GCGCCAAGCT GATCTTCGCC TACGCCGAGG CCACGGTGCC GCTCATCACG
     4201 GTCATCACCC GCAAGGCCTT CGGCGGCGCC TACGACGTCA TGGGCTCCAA GCACCTGGGC
     4261 GCCGACCTCA ACCTGGCCTG GCCCACCGCC CAGATCGCCG TCATGGGCGC CCAAGGCGCG
     4321 GTCAACATCC TGCACCGCCG CACCATCGCC GACGCCGGTG ACGACGCCGA GGCCACCCGG
     4381 GCCCGCCTGA TCCAGGAGTA CGAGGACGCC CTCCTCAACC CCTACACGGC GGCCGAACGC
     4441 GGCTACGTCG ACGCCGTGAT CATGCCCTCC GACACTCGCC GCCACATCGT CCGCGGCCTG
     4501 CGCCAGCTGC GCACCAAGCG CGAGTCCCTG CCCCCGAAGA AGCACGGCAA CATCCCCCTG
     4561 TGAATGCATG AGGGAGCCTC AATCGTGCGC AAGGTGCTCA TCGCCAATCG TGGCGAAATC
     4621 GCTGTCCGCG TGGCCCGGGC CTGCCGGGAC GCCGGGATCG CGAGCGTGGC CGTCTACGCG
     4681 GATCCGGACC GGGACGCGTT GCACGTCCGT GCCGCTGATG AGGCGTTCGC CCTGGGTGGT
     4741 GACACCCCCG CGACCAGCTA TCTGGACATC GCCAAGGTCC TCAAAGCCGC GCGCGAGTCG
     4801 GGCGCGGACG CCATCCACCC CGGCTACGGG TTCCTCTCGG AGAACGCCGA GTTCGCGCAG
     4861 GCGGTCCTGG ACGCCGGCCT GATCTGGATC GGCCCGCCCC CGCACGCCAT CCGCGACCTG
     4921 GGCGACAAGG TCGCCGCCCG CCACATCGCC CAGCGGGCCG GCGCCCCCCT GGTCGCCGGC
     4981 ACCCCCGACC CCGTCTCCGG CGCGGACGAG GTCGTCGCCT TCGCCAAGGA GCACGGCCTG
     5041 CCCATCGCCA TCAAGGCCGC CTTCGGCGGC GGCGGGCGCG GCCTCAAGGT CGCCCGCACC
     5101 CTCGAAGAGG TGCCGGAGCT GTACGACTCC GCCGTCCGCG AGGCCGTGGC CGCCTTCGGC
     5161 CGCGGGGAGT GCTTCGTCGA GCGCTACCTC GACAAGCCCC GCCACGTGGA GACCCAGTGC
     5221 CTGGCCGACA CCCACGGCAA CGTGGTCGTC GTCTCCACCC GCGACTGCTC CCTCCAGCGC
     5281 CGCCACCAAA AGCTCGTCGA GGAGGCCCCC GCGCCCTTCC TCTCCGAGGC CCAGACGGAG
     5341 CAGCTGTACT CATCCTCCAA GGCCATCCTG AAGGAGGCCG GCTACGTCGG CGCCGGCACC
     5401 GTGGAGTTCC TCGTCGGCAT GGACGGCACG ATCTCCTTCC TGGAGGTCAA CACCCGCCTC
     5461 CAGGTCGAGC ACCCGGTCAC CGAGGAAGTC GCCGGCATCG ACCTGGTCCG CGAGATGTTC
     5521 CGCATCGCCG ACGGCGAGGA ACTCGGCTAC GACGACCCCG CCCTGCGCGG CCACTCCTTC
     5581 GAGTTCCGCA TCAACGGCGA GGACCCCGGC CGCGGCTTCC TGCCCGCCCC CGGCACCGTC
     5641 ACCCTCTTCG ACGCGCCCAC CGGCCCCGGC GTCCGCCTGG ACGCCGGCGT CGAGTCCGGC
     5701 TCCGTCATCG GCCCCGCCTG GGACTCCCTC CTCGCCAAAC TGATCGTCAC CGGCCGCACC
     5761 CGCGCCGAGG CACTCCAGCG CGCGGCCCGC GCCCTGGACG AGTTCACCGT CGAGGGCATG
     5821 GCCACCGCCA TCCCCTTCCA CCGCACGGTC GTCCGCGACC CGGCCTTCGC CCCCGAACTC
     5881 ACCGGCTCCA CGGACCCCTT CACCGTCCAC ACCCGGTGGA TCGAGACGGA GTTCGTCAAC
     5941 GAGATCAAGC CCTTCACCAC GCCCGCCGAC ACCGAGACGG ACGAGGAGTC GGGCCGGGAG
     6001 ACGGTCGTCG TCGAGGTCGG CGGCAAGCGC CTGGAAGTCT CCCTCCCCTC CAGCCTGGGC
     6061 ATGTCCCTGG CCCGCACCGG CCTGGCCGCC GGGGCCCGCC CCAAGCGCCG CGCGGCCAAG
     6121 AAGTCCGGCC CCGCCGCCTC GGGCGACACC CTCGCCTCCC CGATGCAGGG CACGATCGTC
     6181 AAGATCGCCG TCGAGGAGGG CCAGGAAGTC CAGGAAGGCG ACCTCATCGT CGTACTCGAG
     6241 GCGATGAAGA TGGAACAGCC CCTCAACGCC CACAGGTCCG GCACCATCAA GGGCCTCACC
     6301 GCCGAGGTCG GCGCCTCCCT CACCTCCGGC GCCGCCATCT GCGAGAATCA CTAGCAACTT
     6361 CATTAAAGAG GAGAAATACT AGATGAAGAT TTACGGAATT TATATGGACC GCCCGCTTTC
     6421 ACAGGAAGAA AATGAACGGT TCATGACTTT CATATCACCT GAAAAACGGG AGAAATGCCG
     6481 GAGATTTTAT CATAAAGAAG ATGCTCACCG CACCCTGCTG GGAGATGTGC TCGTTCGCTC
     6541 AGTCATAAGC AGGCAGTATC AGTTGGACAA ATCCGATATC CGCTTTAGCA CGCAGGAATA
     6601 CGGGAAGCCG TGCATCCCTG ATCTTCCCGA CGCTCATTTC AACATTTCTC ACTCCGGCCG
     6661 CTGGGTCATT GGTGCGTTTG ATTCACAGCC GATCGGCATA GATATCGAAA AAACGAAACC
     6721 GATCAGCCTT GAGATCGCCA AGCGCTTCTT TTCAAAAACA GAGTACAGCG ACCTTTTAGC
     6781 AAAAGACAAG GACGAGCAGA CAGACTATTT TTATCATCTA TGGTCAATGA AAGAAAGCTT
     6841 TATCAAACAG GAAGGCAAAG GCTTATCGCT TCCGCTTGAT TCCTTTTCAG TGCGCCTGCA
     6901 TCAGGACGGA CAAGTATCCA TTGAGCTTCC GGACAGCCAT TCCCCATGCT ATATCAAAAC
     6961 GTATGAGGTC GATCCCGGCT ACAAAATGGC TGTATGCGCC GCACACCCTG ATTTCCCCGA
     7021 GGATATCACA ATGGTCTCGT ACGAAGAGCT TTTATAATAC TAGAGCCAGG CATCAAATAA
     7081 AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
     7141 CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
//
