LOCUS       pRB22                   6334 bp    CIRCULAR             26-AUG-2013
EMBL_ID     pSB1C3+BBa_J04450       6334 bp                         26-AUG-2013
UNIMARK     pRB22_Annotations
            pRB22
FEATURES             Location/Qualifiers
     source          1..6334
                     /comment="Molecule is created with Unipro UGENE v1.12"
     stem_loop       42..147
                     /label="T0 terminator"
     CDS             complement(160..819)
                     /gene="Chloramphenicol resistance"
     primer_bind     933..952
                     /label="Verification forward (VF2) primer binding site"
     stem_loop       complement(1009..1012)
                     /label="end of terminator"
     stem_loop       complement(1022..1041)
                     /label="Stem loop"
     misc_feature    1049..1070
                     /ugene_name="BB_prefix"
     misc_feature    1071..1270
                     /label="BBa_R0010"
     misc_binding    1159..1196
                     /label="CAP"
     promoter        1207..1212
                     /label=-35
     promoter        1231..1236
                     /label=-10
     misc_binding    1236..1270
                     /label="LacI"
     misc_feature    1243..1243
                     /label="start: "
     misc_feature    complement(1265..1299)
                     /mismatches=0
                     /ugene_name="RB22_rv"
                     /ugene_group="Primer"
     misc_feature    1269..1322
                     /mismatches=0
                     /ugene_name="RB27_fw"
                     /ugene_group="Primer"
     misc_feature    1279..1290
                     /label="BBa_B0034"
     misc_feature    1283..1286
                     /label="Conserved: "
     misc_feature    1297..5151
                     /mismatches=0
                     /ugene_name="indC"
                     /ugene_group="gene"
     misc_feature    complement(1478..1522)
                     /mismatches=0
                     /ugene_name="RB69_rv"
                     /ugene_group="Primer"
     misc_feature    1498..1537
                     /mismatches=0
                     /ugene_name="RB70_fw"
                     /ugene_group="Primer"
     misc_feature    complement(1785..1821)
                     /mismatches=0
                     /ugene_name="RB78_rv"
                     /ugene_group="Primer"
     misc_feature    1792..1844
                     /mismatches=0
                     /ugene_name="RB67_fw"
                     /ugene_group="Primer"
     misc_feature    complement(5129..5184)
                     /mismatches=0
                     /ugene_name="RB46_rv"
                     /ugene_group="Primer"
     misc_feature    5155..5172
                     /label="BBa_E1010"
     misc_feature    5155..5189
                     /mismatches=0
                     /ugene_name="RB21_fw"
                     /ugene_group="Primer"
     misc_feature    5173..5197
                     /label="barcode: "
     misc_feature    5206..5285
                     /label="BBa_B0010"
     stem_loop       5217..5260
     misc_feature    5294..5334
                     /label="BBa_B0012"
     stem_loop       5301..5320
                     /label="T7 TE"
     polyA_signal    5321..5334
     misc_feature    5327..5327
                     /label="stop: "
     misc_feature    5335..5355
                     /label="DNA: BioBrick suffix"
     misc_feature    5335..5355
                     /ugene_name="BB_suffix"
     stem_loop       5356..5427
                     /label="E. coli his operon terminator"
     stem_loop       5364..5398
                     /label="Stem loop"
     primer_bind     complement(5491..5510)
                     /label="Verification reverse (VR) primer binding site"
     misc_feature    complement(5595..6209)
                     /gene="rep (pMB1)"
                     /ugene_name="misc"
     misc_feature    5610..5610
                     /gene="ORI"
                     /ugene_name="misc"
ORIGIN
        1 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC TTGGATTCTC ACCAATAAAA
       61 AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT
      121 GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC
      181 ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC
      241 ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC
      301 CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT
      361 GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA
      421 ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG
      481 GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC
      541 GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG
      601 AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
      661 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
      721 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
      781 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
      841 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
      901 GGAACCTCTT ACGTGCCCGA TCAACTCGAG TGCCACCTGA CGTCTAAGAA ACCATTATTA
      961 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT
     1021 CCTTAGCTTT CGCTAAGGAT GATTTCTGGA ATTCGCGGCC GCTTCTAGAG CAATACGCAA
     1081 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
     1141 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
     1201 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
     1261 ATTTCACACA TACTAGAGAA AGAGGAGAAA GGTACCATGT TAGAAAATAA TATTACACAA
     1321 TGTGACTCAA TCAATGATGT TTATCTTAAA GAAGAAGCAA TAACATTGAT GGATATGCTT
     1381 GAGAGTCAAC TTAAGCACCA GGCAGATGGA TATGTTGTTA TTGATCAAGA AGAATCTCTC
     1441 AGTTACGCTG ATTTCTATTT GAGGGTGAAA GAGATAGGGT ATTGTCTGTC AGAAATTAGC
     1501 TCAAAGAGTT CGGTGGGTAT TGGGCTTTTT TGTGATCCTT CTATAGATTT AATTTGTGGT
     1561 GCATGGGGTA TTTTGTCAGC GGATAAAGCT TATTTGCCGT TATCGCCTGA CTATCCAACT
     1621 GAACGCCTCA AATATATGAT AGAAGATTCT GGTATTGATG TGATTTTTAC GCAATCGCAC
     1681 TTAAAAGCAC AGCTACAGGA CATTGCACCA AAATCAGTAT TAATTATGAC ACCAGAAGAT
     1741 GTCGCTCTGA CGATAAAAAC ACGAACAATA GAAGATATTC TGGGCACAGT TCAAGTTCCT
     1801 AAACCCACGA GTCTGGCTTA TATTATTTAT ACCTCTGGTA GCACGGGTAA GCCAAAGGGA
     1861 GTGATGATTG AACATCACAG TATTGTAAAT CAAATGAGAT TTCTTGCAAA AGCGTTCAAA
     1921 TTAGGATGTC ATTCCCGGAT TTTACAGAAA ACACCAATGA GTTTTGATGC GGCTCAATGG
     1981 GAAATTCTAG CGCCTGCAAT TGGTGGTCAA GTGATTATGG GTCCTTTAGG TTGCTATCGC
     2041 GATCCGGATG CAATTATTAA AACCATTCTT CAGCATCAAG TAACGACTTT GCAATGTGTT
     2101 CCTACTTTGC TACAAGCGTT ACTGGATAAT CCTAATTTTT TGGATTGCTT ATCATTGACT
     2161 CAAGTATTCA GTGGGGGAGA AGCGCTGACA ACCAAATTAG CCACGCAATT TTTGAATAGT
     2221 TTTACTCACT GTGAATTAAT CAATTTATAT GGCCCGACAG AATGTACGAT TAATTCATCA
     2281 TTTTTCCGGG TGACAAATGA GACTTTGCCG AATTATCAAA CCTCTATTTC GATTGGTGCA
     2341 CCTGTAGATA ATACCGAATA CTACGTTCTT GATGATGATA GATTACCTGT GGCGGTTGGC
     2401 GAAATTGGCG AGCTTTATAT TTCGGGTGCT CAATTAGCAC GTGGTTATTT GCATAAACCA
     2461 GAAATGACAA AAGATAAATT TATTTGTAAT CACCTTGTAT CAGGAACTCA ACATCAATGG
     2521 TTATATCGAA CGGGAGATCT GGTAACCAGA GGGGCTGATG GTAATACTTA TTTTGTTGGT
     2581 CGGGTTGATA GCCAGGTCAA ATTACGAGGT TACCGTATTG AGCTTGATGA AATACGCCAT
     2641 GCGATTGAAG AACATAGCTG GATAAAGACG GCGGCAATGT TAATTAAGAA GGATGCCAGA
     2701 ACGGGTTTCC AAAATCTCAT CGCGTGTGTG GAATTAGATG AGAAAGAAGC TGCATTGATG
     2761 GATCAAGGTA ATAGTAGCTC ACATCACAAA TCAAAAGCCG ATAAACTACA GGTGAAAGCC
     2821 CAACTTTCTA ATTCTGGTTG TCGAAGTGAA GAGTTATGTG AAAATCGCCC TACATTCTTA
     2881 CTTCCTTATC AAGAAGGGGA GATAAAACAG AGAGAATATG CATTTGGACG CAAGACATAT
     2941 CGCTATTTTG AGGGAACAGA AATAACGGTA GAGAAATTAA AAAAATTGCT GACAGCCACT
     3001 CAATCGAATG AAATTAGCTC TTTGCCACTG AGTCATCTAA CCCTGAATGA TTTCGGTTAT
     3061 GCATTGCGTT ATTTTGGTCA GTTTACCAGC CATCAACGTT TATTGCCCAA ATATGCCTAT
     3121 GCTTCACCGG GTGCTCTCTA TGCGACACAA ATGTATTTTG AATTGCATAA TGTTCTCGGT
     3181 TTGGATGCGG GGATTTACTA TTATCATCCA GTGACACATA AGTTAATAAA AATTTCAACA
     3241 TTGAGTCGTC GGCAAATGCC AACGATAAAA GTGCATTTTA TTGGCAAGCA TGAAGCCATT
     3301 GAGCCCGTTT ATAAGAACAA TATACAAGAA GTTCTGGAAA TGGAAGCGGG CCATATGATG
     3361 GGTCTTTTTG ATGACGTATT ACCGGAAATT GGCTTGAGTA TTGGTAAAAG TGAATATCAA
     3421 GATGAATGTC CAGATTGGTA TGATGGTGAT ATTCAGGATT ATTATCTTGG TGCATTTGAA
     3481 ATATGTAGCT ATGAACATGG ATTGCCGCCA TTTGAGACTG ATATTTATTT ACAAACACAT
     3541 GCCCATAAAA TACCTGAGAT GCCGTGTGGT TTATATCACT TTTCTAACGG GGAATTTGTA
     3601 CGAATAAGTG ATGATATTGT CCGAAAAAAG GATGTTATTG CGATTAATCA GCAAGTTTAT
     3661 GATCGCTCCA GTTTTGGCGT GTCAATTATT CCACGCTGTG TCCCTGAATG GCATTATTAT
     3721 ATAACACTGG GTCGTCGGTT ACATGCGTTA CAAAGTAATC CATTGTATAT TGGATTAATG
     3781 TCATCTGGTT ACAGTTCGAA GAGCAATAAC GATTTACCTT CGGCGAAAAG GATGCGATCT
     3841 ATTCTCAATG CACTTGATAG ACCTATGGCG GCATTTTATT TCTGCATAGG TGGGGGTATT
     3901 AGCCAAGCGC AATATATGTG TGAAGGCATG AAAGAAGATG TTGTTCATAT GAAAGGGCCA
     3961 GTTGAAATCA TTAAAGATGA TCTTCAACAA CAACTCCCTC AATATATGAT TCCAAATAAG
     4021 GTATTAGTTT TCGATAAATT ACCTTTGACG GCCAATGGAA AAGTGGATTA TCAATCTTTA
     4081 TCAGAATCTA AAGCCGTGGA GAATGTTTCA ACACAGCGTC TATTGGTGCC ATTACATACA
     4141 GATACTGAAA TAAGGCTTGG AAAAATTTGG ATGGAAGTAC TGAAATGGGA TTCAGTATCT
     4201 GCCCTCGATG ATTTTTTCGA AAGTGGGGGT AATTCTTTGA TGGCCGTTGC AATGGTTAAT
     4261 AAGATCAATG CGGCCTTTAA TATTCGTTTT CCGTTACAGA TACTTTTTCA ATCTCCTAAT
     4321 ATAGCAGAAT TGGCTAAGTG GATTGAACAG ACAGACTCTA AAACAATATC AAGATTAATT
     4381 TTATTGAATC AGGCAAGCAA AGACCCCATT TACTGTTGGC CGGGTTTGGG CGGATATCCT
     4441 ATGAGTTTGA GATTGCTTGC TAATAAAGTC GTTCCTGATC GGGCATTTTA TGGAATACAG
     4501 GCATATGGGA TAAACGAGAG TGAAATACCG TTTTCTTCTA TCCAGAGAAT GGCAGAAGAG
     4561 GATATTAAAG AGATAAAGAA AATACAGCCA GAAGGGCCAT ATATATTGTG GGGATATTCA
     4621 TTTGGTGCCC GAGTAGCATT TGAAGTTGCA TACCAGCTTG AACAAGCGGG AGAAGAAGTT
     4681 AACGCATTGA ATTTATTGGC TCCGGGATCT CCTCATCTTG ATATGAAGCA AGCGGAATAT
     4741 ATGGATAAAG GCGCTGAATT TACTAATCCG GCTTTTGTTA AAATACTTTT TTCTGTATTT
     4801 TCTCGTTCAA TCAACAGCCC AATGGTTAAA ACTTGCTTAG AACAAGTAAA TAGTGAAACG
     4861 ACATTTATTA ACTTTATATG TAGTCGTTTT AAAAACTTGG AACCATCATT AGTAAAACGT
     4921 ATCGTTAGGA TTGTGACTTT GACTTATGAT TTCAAGTACA GTATTGATGA GCTTTATCAC
     4981 AGACACCTAA AGGCACCTAT AACTATTTTC AAGGCGAATA GAGATAATGA TTCATTTATC
     5041 GAGGAATCGG ATGTGATTTC ATCAATGTCG CCTAAAATAA TTGAATTAAT ATCGGATCAC
     5101 TATCAACTGT TGGAAAGTGA AGGTGTTGCT GAGATTGAGA AAATAATCTA ATAATAATGA
     5161 GCTAGCTAAT AACGCTGATA GTGCTAGTGT AGATCGCTAC TAGAGCCAGG CATCAAATAA
     5221 AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
     5281 CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
     5341 TAGCGGCCGC TGCAGTCCGG CAAAAAAGGG CAAGGTGTCA CCACCCTGCC CTTTTTCTTT
     5401 AAAACCGAAA AGATTACTTC GCGTTATGCA GGCTTCCTCG CTCACTGACT CGCTGCGCTC
     5461 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     5521 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     5581 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCACAGGCTC CGCCCCCCTG ACGAGCATCA
     5641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     5701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     5761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     5821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     5881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     5941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     6001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     6061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     6121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     6181 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     6241 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     6301 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATC
//
