LOCUS       new_mol1                8200 bp    CIRCULAR             12-AUG-2013
EMBL_ID     new_mol1                8200 bp    CIRCULAR             04-AUG-2013
EMBL_ID     new_mol1                8200 bp    CIRCULAR             04-AUG-2013
EMBL_ID     new_mol1                8200 bp    CIRCULAR             12-AUG-2013
UNIMARK     new_mol1 annotations
            new_mol1
FEATURES             Location/Qualifiers
     source          1..8200
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    join(1..15,8195..8200)
                     /label="DNA: BioBrick suffix"
     stem_loop       16..87
                     /label="E. coli his operon terminator"
     stem_loop       24..58
                     /label="Stem loop"
     primer_bind     complement(151..170)
                     /label="Verification reverse (VR) primer binding site"
     misc_feature    complement(255..869)
                     /gene="rep (pMB1)"
                     /ugene_name="misc"
     misc_feature    270..270
                     /gene="ORI"
                     /ugene_name="misc"
     stem_loop       1036..1141
                     /label="T0 terminator"
     CDS             complement(1154..1813)
                     /gene="Chloramphenicol resistance"
     primer_bind     1927..1946
                     /label="Verification forward (VF2) primer binding site"
     stem_loop       complement(2003..2006)
                     /label="end of terminator"
     stem_loop       complement(2016..2035)
                     /label="Stem loop"
     misc_feature    2045..2064
                     /label="DNA: BioBrick prefix"
     misc_feature    2065..2264
                     /label="BBa_R0010"
     misc_binding    2153..2190
                     /label="CAP"
     promoter        2201..2206
                     /label=-35
     promoter        2225..2230
                     /label=-10
     misc_binding    2230..2264
                     /label="LacI"
     misc_feature    2277..2280
                     /label="Conserved: "
     misc_feature    2291..4153
                     /ugene_name="TycB1-dCom-dC"
     misc_feature    4154..8026
                     /ugene_name="TycC6"
     misc_feature    8033..8057
                     /label="barcode: "
     misc_feature    8066..8145
                     /label="BBa_B0010"
     stem_loop       8077..8120
     misc_feature    8154..8194
                     /label="BBa_B0012"
     stem_loop       8161..8180
                     /label="T7 TE"
     polyA_signal    8181..8194
     misc_feature    8187..8187
                     /label="stop: "
ORIGIN
        1 TAGCGGCCGC TGCAGTCCGG CAAAAAAGGG CAAGGTGTCA CCACCCTGCC CTTTTTCTTT
       61 AAAACCGAAA AGATTACTTC GCGTTATGCA GGCTTCCTCG CTCACTGACT CGCTGCGCTC
      121 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
      181 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
      241 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCACAGGCTC CGCCCCCCTG ACGAGCATCA
      301 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
      361 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
      421 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
      481 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
      541 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
      601 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
      661 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
      721 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
      781 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
      841 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
      901 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
      961 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1021 TGACAGCTCG AGGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT
     1081 GAACAAATCC AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA
     1141 GCTCGATATC AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA
     1201 GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA
     1261 TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT
     1321 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA
     1381 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG
     1441 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA
     1501 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC
     1561 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC
     1621 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA
     1681 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA
     1741 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
     1801 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC
     1861 CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CCGATCAACT
     1921 CGAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
     1981 CGTATCACGA GGCAGAATTT CAGATAAAAA AAATCCTTAG CTTTCGCTAA GGATGATTTC
     2041 TGGAATTCGC GGCCGCTTCT AGAGCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
     2101 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
     2161 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
     2221 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACATACTAG AGAAAGAGGA
     2281 GAAATACTAG ATGGATTGCG TGGCAAACAA TTCGGGAGTC GAGCTTTGCC AGATTCCGTT
     2341 GCTGACAGAA GCAGAAACTA GCCAGCTGTT GGCAAAGCGT ACGGAAACAG CGGCTGACTA
     2401 TCCTGCCGCA ACCATGCACG AGCTGTTTTC GCGGCAGGCA GAAAAAACGC CTGAGCAAGT
     2461 GGCGGTAGTC TTCGCGGATC AGCACCTGAC GTATCGGGAG CTGGATGAAA AATCCAATCA
     2521 GCTCGCCCGC TTTTTGCGCA AAAAAGGCAT TGGCACGGGC AGTCTTGTCG GCACGCTGCT
     2581 GGATCGCTCG CTGGACATGA TCGTCGGAAT CCTCGGCGTC TTGAAGGCAG GCGGCGCATT
     2641 TGTGCCGATC GACCCGGAGT TGCCTGCCGA ACGAATCGCT TACATGCTGA CGCATAGCAG
     2701 AGTTCCATTG GTCGTGACGC AAAATCATTT GCGGGCAAAA GTGACCACGC CTACAGAAAC
     2761 AATTGACATC AACACAGCGG TGATCGGGGA AGAGAGCCGC GCCCCTATCG AATCGCTCAA
     2821 TCAGCCGCAT GACTTGTTTT ACATCATCTA TACGTCCGGA ACGACAGGGC AACCGAAAGG
     2881 CGTCATGCTG GAGCATCGCA ACATGGCGAA CCTGATGCAT TTTACGTTTG ATCAGACGAA
     2941 CATCGCTTTT CATGAAAAAG TGTTGCAGTA TACCACGTGC AGCTTTGATG TTTGCTACCA
     3001 GGAAATTTTC TCCACGCTGC TATCCGGGGG CCAGCTCTAC CTGATCACGA ACGAGCTGAG
     3061 ACGGCATGTG GAAAAGCTGT TTGCTTTCAT CCAGGAAAAG CAGATCAGCA TTTTGTCTCT
     3121 CCCGGTGTCC TTCCTGAAAT TTATTTTTAA CGAACAAGAC TACGCGCAAA GCTTCCCGCG
     3181 TTGTGTCAAA CATATCATCA CGGCCGGGGA ACAACTCGTC GTCACACACG AGCTGCAAAA
     3241 GTATCTGCGC CAGCATCGCG TATTTTTGCA CAATCACTAC GGCCCGTCGG AGACGCATGT
     3301 GGTGACGACA TGCACGATGG ACCCGGGACA GGCGATACCA GAGCTGCCGC CCATCGGAAA
     3361 GCCGATCAGC AACACAGGCA TTTACATTTT GGATGAAGGG CTGCAATTGA AGCCGGAGGG
     3421 GATCGTCGGG GAGTTGTACA TTTCCGGCGC AAACGTAGGA AGAGGGTATT TGCACCAGCC
     3481 GGAGCTGACC GCGGAGAAGT TTCTCGACAA TCCGTATCAG CCAGGCGAAA GAATGTACCG
     3541 AACGGGTGAT CTGGCCCTTT GGTTGCCGGA TGGCCAGCTC GAATTTTTGG GCCGAATCGA
     3601 CCATCAGGTA AAAATCAGGG GCCATCGCAT CGAGCTGGGA GAGATCGAAT CGCGCCTGCT
     3661 CAACCATCCC GCCATCAAGG AAGCGGTGGT TATCGACCGA GCAGACGAGA CAGGCGGCAA
     3721 GTTTTTGTGC GCCTATGTCG TCCTGCAAAA AGCGCTCAGC GACGAAGAGA TGCGGGCATA
     3781 CTTGGCGCAA GCGTTGCCGG AGTATATGAT CCCTTCCTTT TTCGTGACGC TGGAGCGGAT
     3841 TCCAGTCACG CCGAACGGAA AAACAGACAG GCGAGCTTTG CCGAAGCCGG AAGGAAGTGC
     3901 CAAGACGAAA GCGGATTACG TCGCCCCGAC GACTGAGCTG GAACAAAAGC TGGTCGCGAT
     3961 TTGGGAGCAA ATTCTTGGCG TGTCGCCGAT CGGCATTCAG GATCATTTTT TCACGCTGGG
     4021 CGGCCATTCG TTAAAAGCGA TTCAGCTCAT TTCCCGCATC CAAAAGGAAT GCCAGGCGGA
     4081 TGTCCCGCTG CGCGTCCTGT TTGAGCAACC GACGATTCAA GCGCTGGCAG CGTATGTGGA
     4141 AGGCGGGGAG GAAGGGAATG TCTTCTCGAT CGAGCCTGTG CAAAAGCAAG CGTACTATCC
     4201 GGTCTCCTCG GCACAAAAGC GCATGTACAT CCTCGATCAA TTTGAGGGAG TCGGCATCAG
     4261 CTACAACATG CCGTCGACTA TGCTGATCGA AGGCAAGCTG GAGCGAACAC GGGTAGAAGC
     4321 GGCGTTCCAG CGCTTGATTG CGCGACATGA AAGCCTGCGC ACTTCGTTTG CCGTCGTCAA
     4381 CGGAGAGCCT GTGCAAAACA TTCACGAGGA CGTTCCGTTT GCGCTTGCCT ATTCGGAAGT
     4441 CACAGAACAG GAGGCGCGCG AACTCGTTTC TTCTCTCGTG CAGCCGTTCG ATCTGGAGGT
     4501 CGCACCACTC ATCCGCGTGT CGCTGCTGAA AATCGGCGAG GATCGTTACG TGCTCTTTAC
     4561 CGACATGCAT CACAGCATTT CCGATGGCGT ATCCTCCGGC ATTCTTTTGG CAGAGTGGGT
     4621 GCAGCTGTAC CAGGGTGACG TTTTGCCGGA GCTGCGTATC CAGTACAAGG ACTTTGCTGT
     4681 GTGGCAACAA GAGTTTTCCC AGTCGGCTGC CTTCCACAAG CAGGAAGCGT ACTGGTTGCA
     4741 AACGTTTGCC GATGACATTC CTGTGCTGAA CTTGCCGACC GATTTCACCC GCCCCAGCAC
     4801 CCAAAGCTTT GCCGGGGATC AGTGCACGAT CGGCGCGGGC AAAGCGCTCA CGGAAGGCTT
     4861 GCACCAGTTG GCGCAGGCGA CGGGAACGAC TTTGTACATG GTTTTGCTCG CCGCGTACAA
     4921 CGTGCTGCTC GCCAAGTATG CCGGGCAGGA GGACATCATC GTCGGCACGC CGATTACAGG
     4981 CAGATCCCAT GCCGATCTCG AACCGATCGT CGGCATGTTC GTGAACACCT TGGCGATGCG
     5041 AAACAAACCG CAGCGCGAAA AGACTTTTAG CGAGTTTTTG CAAGAAGTCA AGCAAAATGC
     5101 GCTGGATGCG TACGGCCATC AGGATTACCC GTTTGAAGAA CTGGTGGAAA AGCTCGCGAT
     5161 CGCGCGCGAT TTGAGCCGAA ATCCGCTGTT TGACACCGTG TTTACGTTCC AAAACAGCAC
     5221 GGAAGAGGTC ATGACGCTGC CTGAATGCAC GCTTGCGCCG TTTATGACGG ACGAAACAGG
     5281 CCAGCACGCC AAGTTCGACT TGACTTTCAG CGCTACGGAA GAGCGGGAAG AAATGACGAT
     5341 TGGCGTGGAG TACAGCACAA GCTTGTTTAC GCGGGAAACG ATGGAACGGT TCAGCCGCCA
     5401 CTTCCTGACG ATTGCAGCGA GCATCGTGCA AAATCCGCAC ATCCGTCTGG GCGAGATCGA
     5461 CATGCTTTTG CCAGAAGAAA AACAGCAGAT TTTGGCCGGG TTCAACGATA CGGCAGTCAG
     5521 CTATGCGCTG GACAAAACGC TGCACCAGCT ATTCGAAGAG CAGGTCGACA AAACACCGGA
     5581 TCAGGCAGCG CTTCTCTTTA GCGAGCAATC GCTGACGTAC AGCGAACTGA ACGAGCGAGC
     5641 AAACAGACTG GCAAGGGTCC TGCGCGCAAA AGGAGTCGGA CCGGACCGTC TGGTAGCGAT
     5701 CATGGCGGAG CGCTCGCCGG AAATGGTGAT CGGTATTCTC GGTATTTTGA AGGCAGGCGG
     5761 CGCTTATGTT CCCGTCGATC CCGGCTATCC GCAGGAGCGC ATTCAGTACC TGCTCGAAGA
     5821 TAGCAACGCA GCCCTGCTGC TCAGCCAGGC GCATCTGTTG CCGCTGTTGG CCCAGGTGTC
     5881 AAGCGAGCTG CCGGAGTGCC TTGATCTGAA CGCTGAACTG GATGCCGGAC TGAGCGGCTC
     5941 CAACCTGCCA GCTGTCAACC AACCGACTGA CCTTGCCTAC GTCATCTATA CATCCGGTAC
     6001 GACCGGCAAG CCGAAGGGTG TCATGATCCC GCATCAAGGA ATCGTGAACT GCTTGCAGTG
     6061 GAGAAGAGAC GAATACGGGT TCGGGCCGAG TGACAAGGCG TTGCAAGTGT TCTCCTTTGC
     6121 CTTCGACGGT TTTGTAGCCA GCTTGTTCGC TCCGCTGCTC GGAGGGGCAA CGTGCGTGTT
     6181 GCCGCAAGAA GCAGCTGCCA AAGACCCGGT CGCGCTGAAA AAACTGATGG CCGCAACGGA
     6241 AGTCACCCAT TACTACGGCG TACCGAGTCT GTTCCAGGCC ATTCTCGATT GCTCGACGAC
     6301 AACCGACTTC AATCAGTTGC GTTGCGTCAC TTTGGGCGGC GAGAAGCTGC CTGTGCAGCT
     6361 TGTGCAAAAA ACAAAAGAAA AGCATCCGGC AATCGAGATC AACAACGAGT ACGGCCCGAC
     6421 GGAAAACAGC GTCGTCACCA CCATCTCGCG CTCGATTGAA GCGGGGCAAG CGATCACGAT
     6481 TGGCCGACCG CTTGCGAACG TCCAAGTCTA CATTGTAGAT GAGCAGCATC ACTTGCAGCC
     6541 GATTGGCGTG GTCGGTGAGC TGTGCATCGG CGGAGCCGGG CTTGCCAGAG GCTATCTGAA
     6601 CAAACCGGAG CTGACCGCAG AGAAGTTTGT CGCAAATCCG TTCCGACCAG GCGAGCGCAT
     6661 GTACAAAACA GGCGACTTGG TAAAATGGCG GACGGATGGC ACGATCGAGT ACATCGGCCG
     6721 CGCAGACGAA CAGGTCAAGG TGAGAGGGTA TCGCATCGAG ATCGGCGAGA TCGAGAGCGC
     6781 CGTACTCGCT TACCAGGGCA TCGATCAAGC GGTGGTCGTT GCGCGAGACG ATGACGCTAC
     6841 GGCTGGTTCC TATCTTTGCG CCTACTTTGT CGCAGCAACA GCCGTGTCCG TATCCGGCTT
     6901 GAGAAGCCAT CTGGCCAAAG AGCTGCCTGC TTACATGATT CCGAGCTATT TCGTCGAGCT
     6961 GGATCAGCTG CCGCTTTCCG CCAATGGAAA AGTGGATCGC AAAGCTTTGC CGAAGCCGCA
     7021 ACAGTCCGAT GCGACCACGC GCGAATACGT GGCCCCGAGG AATGCGACCG AACAGCAACT
     7081 GGCAGCCATC TGGCAAGAAG TTTTGGGAGT AGAGCCAATC GGCATCACCG ACCAGTTCTT
     7141 TGAACTCGGA GGACATTCCT TAAAAGCTAC GCTGTTGATT GCCAAAGTGT ATGAGTACAT
     7201 GCAAATCGAG CTGCCGCTGA ATCTCATCTT CCAGTATCCG ACGATCGAAA AGGTGGCCGA
     7261 TTTCATCACG CATAAGCGCT TTGAGAGCAG ATACGGCACA GCCATTTTGT TAAATCAGGA
     7321 GACGGCGCGA AACGTATTTT GCTTCACGCC GATCGGCGCA CAAAGCGTGT ACTACCAGAA
     7381 GCTTGCGGCG GAAATTCAAG GCGTCTCTTT GTACAGCTTT GATTTCATCC AGGATGACAA
     7441 CCGGATGGAG CAGTATATCG CGGCGATCAC CGCAATTGAT CCAAGCGGTC CGTACACGCT
     7501 CATGGGCTAC TCCTCGGGAG GCAATCTGGC TTTTGAAGTG GCGAAAGAAC TGGAGGAGCG
     7561 GGGCTATGGC GTCACCGACA TCATCTTGTT CGACTCGTAC TGGAAAGACA AGGCGATTGA
     7621 GCGGACTGTC GCGGAAACAG AAAACGACAT TGCCCAGCTA TTCGCCGAGA TTGGCGAAAA
     7681 CACCGAGATG TTCAACATGA CGCAAGAAGA CTTCCAGCTG TACGCCGCCA ATGAGTTTGT
     7741 CAAGCAAAGC TTCGTTCGCA AAACGGTCAG CTATGTGATG TTCCATAACA ATCTGGTCAA
     7801 TACCGGAATG ACCACTGCCG CGATCCACCT CATCCAATCC GAGCTGGAAG CAGACGAGGA
     7861 AGCTCCGGTG GCAGCCAAGT GGAACGAATC AGCCTGGGCA AACGCAACGC AACGACTGCT
     7921 GACGTACAGC GGGCACGGAA TCCACTCGCG CATGCTGGCG GGCGATTACG CGTCGCAAAA
     7981 TGCTTCGATT TTGCAAAACA TCCTGCAAGA ACTGTTCATC CTGAAATAAT AACGCTGATA
     8041 GTGCTAGTGT AGATCGCTAC TAGAGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     8101 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT
     8161 GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
//
