LOCUS pRB16 3897 bp CIRCULAR 05-SEP-2013 EMBL_ID pRB16 3897 bp 05-SEP-2013 UNIMARK pRB16_Annotations pRB16 FEATURES Location/Qualifiers source 1..3897 /comment="Molecule is created with Unipro UGENE v1.12" misc_feature 2..73 /gene="E. coli his operon terminator" /ugene_name="misc" stem_loop 10..44 /label="terminator stem loop" misc_feature 14..15 /gene="deletion of two bases" /ugene_name="misc" primer_bind complement(137..156) /label="VR" CDS complement(219..1034) /gene="kanR (rev)" CDS complement(1366..2190) /gene="p15A ORI (rev)" primer_bind 2591..2610 /label="VF2" misc_feature complement(2667..2670) /gene="end of terminator" /ugene_name="misc" stem_loop complement(2680..2699) /label="terminator stem loop" misc_feature 2731..2930 /label="BBa_R0010" misc_binding 2819..2856 /label="CAP" promoter 2867..2872 /label=-35 promoter 2891..2896 /label=-10 misc_binding 2896..2930 /label="LacI" misc_feature 2903..2903 /label="start: " misc_feature complement(2913..2962) /mismatches=0 /ugene_name="RB63_rv" /ugene_group="Primer" misc_feature 2943..2974 /mismatches=0 /ugene_name="RB29_fw" /ugene_group="Primer" misc_feature 2960..3703 /mismatches=0 /ugene_name="svp" /ugene_group="gene" misc_feature complement(3685..3720) /mismatches=0 /ugene_name="RB30_rv" /ugene_group="Primer" misc_feature 3698..3732 /mismatches=0 /ugene_name="RB21_fw" /ugene_group="Primer" misc_feature 3721..3740 /label="barcode: " misc_feature 3749..3828 /label="BBa_B0010" stem_loop 3760..3803 misc_feature 3837..3877 /label="BBa_B0012" stem_loop 3844..3863 /label="T7 TE" polyA_signal 3864..3877 misc_feature 3870..3870 /label="stop: " ORIGIN 1 GTCCGGCAAA AAAACGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG 61 ATTACTTCGC GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATCTCG AGTCCCGTCA AGTCAGCGTA 181 ATGCTCTGCC AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC 241 AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT 301 TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT 361 CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA 421 ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA 481 AGCTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA 541 TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG 601 CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT 661 GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT 721 GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC 781 TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA 841 ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC 901 CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC 961 CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT 1021 TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT 1081 CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG 1141 CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC 1201 AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC 1261 AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC 1321 TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTAGCG GAGTGTATAC 1381 TGGCTTACTA TGTTGGCACT GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA 1441 AAAGGCTGCA CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT 1501 CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT ACGAACGGGG 1561 CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG GAAGTGAGAG GGCCGCGGCA 1621 AAGCCGTTTT TCCATAGGCT CCGCCCCCCT GACAAGCATC ACGAAATCTG ACGCTCAAAT 1681 CAGTGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCT GGCGGCTCCC 1741 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC 1801 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC 1861 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 1921 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT 1981 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG 2041 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT 2101 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC 2161 AAAACGATCT CAAGAAGATC ATCTTATTAA GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 2221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 2281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 2341 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 2401 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 2461 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 2521 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 2581 TATTCCATGG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA 2641 AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT 2701 GATTTCTGGA ATTCGCGGCC GCTTCTAGAG CAATACGCAA ACCGCCTCTC CCCGCGCGTT 2761 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 2821 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 2881 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA TACTAGAGTT 2941 CACACAGGAA ACCGGATCCA TGATCGCCGC CCTCCTGCCC TCCTGGGCCG TCACCGAACA 3001 CGCCTTCACC GACGCCCCGG ACGACCCGGT GAGCCTCCTC TTCCCCGAGG AGGCCGCCCA 3061 CGTCGCCCGC GCCGTCCCCA AGCGCCTGCA CGAGTTCGCC ACCGTCCGGG TGTGCGCCCG 3121 CGCCGCCCTC GGCCGGCTGG GCCTCCCGCC CGGTCCGCTG CTGCCCGGCC GACGGGGCGC 3181 GCCGAGCTGG CCGGACGGGG TGGTGGGGAG CATGACGCAC TGTCAGGGCT TCCGGGGCGC 3241 CGCGGTCGCC CGGGCCGCCG ACGCCGCGTC GCTCGGGATA GACGCCGAGC CGAACGGGCC 3301 GCTCCCGGAC GGCGTCCTCG CCATGGTCTC GCTGCCGTCC GAGCGCGAGT GGCTCGCCGG 3361 ACTGGCGGCC CGCCGGCCGG ACGTGCACTG GGACCGGCTG CTGTTCAGCG CCAAGGAGAG 3421 CGTCTTCAAG GCGTGGTACC CGCTGACCGG CCTGGAGCTG GACTTCGACG AGGCCGAGCT 3481 GGCCGTCGAT CCGGACGCCG GGACGTTCAC GGCCCGGCTG CTGGTGCCGG GACCGGTGGT 3541 CGGCGGCCGT CGGCTGGACG GGTTCGAGGG GCGCTGGGCG GCGGGCGAGG GCCTCGTCGT 3601 CACGGCCATC GCCGTCGCGG CGCCGGCCGG TACCGCGGAG GAATCGGCGG AAGGGGCCGG 3661 GAAGGAAGCG ACTGCGGACG ACCGGACCGC CGTCCCGTAA TGAGCTAGCT AATAACGCTG 3721 ATAGTGCTAG TGTAGATCGC TACTAGAGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG 3781 AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC 3841 ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATATAC TAGTAGCGGC CGCTGCA //