LOCUS new_mol1 7188 bp CIRCULAR 25-JUL-2013 EMBL_ID new_mol1 7188 bp CIRCULAR 19-JUL-2013 EMBL_ID new_mol1 7175 bp CIRCULAR 16-JUL-2013 EMBL_ID new_mol1 7181 bp CIRCULAR 16-JUL-2013 EMBL_ID new_mol1 7188 bp CIRCULAR 19-JUL-2013 UNIMARK new_mol1 annotations new_mol1 FEATURES Location/Qualifiers misc_feature 1..21 /note="BioBrick cloning site suffix (BBa_G00001)" misc_feature 35..46 /note="translational stop sequence for BioBrick vector-insert insulation, has stop codons in all 6 B0042)" frames (BBa_B0042)" terminator 47..115 /note="derived from tonB terminator (BBa_B0054); similar to Escherichia coli strain MG1655 accession U00096" stem_loop 57..88 /note="derived from tonB terminator, predicted using mFold" primer_bind complement(116..135) /note="VR reverse sequencing primer annealing site (BBa_G00102)" terminator 136..176 /note="derived from ribosomal RNA rrnC operon, reverse orientation (BBa_B0062); similar to Escherichia coli ion U00096"55 accession U00096" stem_loop complement(149..168) /note="derived from ribosomal RNA rrnC operon" rep_origin 184..1737 /note="p15A low to medium copy replication origin (BBa_I50032)" rep_origin 582..1406 /note="p15A replication origin" gene 1852..2511 /gene="cat" CDS 1852..2511 /gene="cat" /note="confers resistance to chloramphenicol" /codon_start=1 /transl_table=11 /product="chloramphenicol acetyl transferase" /protein_id="ACB05498.1" /db_xref="GI:169921171" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" terminator 2522..2593 /note="derived from hisI terminator (BBa_B0053); similar to Escherichia coli strain MG1655 accession U00096" stem_loop 2530..2564 /note="derived from hisI terminator" primer_bind 2594..2613 /note="VF2 forward sequencing primer annealing site (BBa_G00100)" terminator 2614..2691 /note="derived from bacteriophage T3 terminator, reverse orientation (BBa_B0055); similar to bacteriophage T3 gene ion number X17255)"ssion number X17255)" stem_loop complement(2649..2670) /note="derived from bacteriophage T3 terminator" misc_feature 2692..2703 /note="translational stop sequence for BioBrick vector-insert insulation, has stop codons in all 6 B0042)" frames (BBa_B0042)" misc_feature 2717..2738 /note="BioBrick cloning site prefix (BBa_G00000)" misc_feature 2739..2938 /label="BBa_R0010" misc_binding 2827..2864 /label="CAP" promoter 2875..2880 /label=-35 promoter 2899..2904 /label=-10 misc_binding 2904..2938 /label="LacI" misc_feature 2911..2911 /label="start: " misc_feature complement(2941..2996) /mismatches=0 /ugene_name="IK35" /ugene_group="Primer" misc_feature 2947..2958 /label="BBa_B0034" misc_feature 2951..2954 /label="Conserved: " misc_feature 2965..4557 /source="Streptomyces coelicolor A3(2)" /ugene_name="pccB" /ugene_group="gene" misc_feature 2965..2983 /mismatches=0 /ugene_name="IK34" /ugene_group="Primer" misc_feature 4558..6348 /source="Streptomyces coelicolor A3(2)" /ugene_name="accA2" /ugene_group="gene" misc_feature 4579..4581 /locus_ref="accA2" /ugene_name="alternate startcodon" misc_feature 6319..6403 /mismatches=0 /ugene_name="IK30" /ugene_group="Primer" misc_feature complement(6328..6359) /mismatches=0 /ugene_name="IK29" /ugene_group="Primer" misc_feature 6341..6341 /label="putative" /ugene_name="insertion" misc_feature 6356..6370 /mismatches=0 /ugene_name="BBa_B0030" /ugene_group="RBS" gene 6377..7051 /gene="sfp gene" CDS 6377..7051 /gene="sfp gene" /codon_start=1 /transl_table=11 /protein_id="CAA44858.1" /db_xref="GI:40139" /db_xref="GOA:P39135" /db_xref="InterPro:IPR004568" /db_xref="InterPro:IPR008278" /db_xref="PDB:1QR0" /db_xref="PDB:2GE0" /db_xref="PDB:2GE1" /db_xref="UniProtKB/Swiss-Prot:P39135" /translation="MKIYGIYMDRPLSQEENERFMTFISPEKREKCRRFYHKEDAHRT LLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPCIPDLPDAHFNISHSGRWVIGAFDSQ PIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKDEQTDYFYHLWSMKESFIKQEGKG LSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEVDPGYKMAVCAAHPDFPEDITMV SYEELL" misc_feature 7019..7078 /mismatches=0 /ugene_name="IK32" /ugene_group="Primer" misc_feature 7060..7139 /label="BBa_B0010" stem_loop 7071..7114 misc_feature 7148..7188 /label="BBa_B0012" stem_loop 7155..7174 /label="T7 TE" polyA_signal 7175..7188 misc_feature 7181..7181 /label="stop: " ORIGIN 1 TACTAGTAGC GGCCGCTGCA GGAGTCACTA AGGGTTAGTT AGTTAGATTA GCAGAAAGTC 61 AAAAGCCTCC GACCGGAGGC TTTTGACTAA AACTTCCCTT GGGGTTATCA TTGGGGCTCA 121 CTCAAAGGCG GTAATCAGAT AAAAAAAATC CTTAGCTTTC GCTAAGGATG ATTTCTGCTA 181 GAGATGGAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 241 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 301 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 361 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 421 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 481 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 541 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CTTAATAAGA TGATCTTCTT 601 GAGATCGTTT TGGTCTGCGC GTAATCTCTT GCTCTGAAAA CGAAAAAACC GCCTTGCAGG 661 GCGGTTTTTC GAAGGTTCTC TGAGCTACCA ACTCTTTGAA CCGAGGTAAC TGGCTTGGAG 721 GAGCGCAGTC ACCAAAACTT GTCCTTTCAG TTTAGCCTTA ACCGGCGCAT GACTTCAAGA 781 CTAACTCCTC TAAATCAATT ACCAGTGGCT GCTGCCAGTG GTGCTTTTGC ATGTCTTTCC 841 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGACTG AACGGGGGGT 901 TCGTGCATAC AGTCCAGCTT GGAGCGAACT GCCTACCCGG AACTGAGTGT CAGGCGTGGA 961 ATGAGACAAA CGCGGCCATA ACAGCGGAAT GACACCGGTA AACCGAAAGG CAGGAACAGG 1021 AGAGCGCACG AGGGAGCCGC CAGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 1081 CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG 1141 AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA 1201 GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA 1261 GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG 1321 GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC 1381 ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT 1441 GACTCATACC AGGCCTGAAT CGCCCCATCA TCCAGCCAGA AAGTGAGGGA GCCACGGTTG 1501 ATGAGAGCTT TGTTGTAGGT GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA 1561 CGGTCTGCGT TGTCGGGAAG ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT 1621 ATTCAACAAA GCCACGTTGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT 1681 ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTTAC 1741 TAGAGGTTGA TCGGGCACGT AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA 1801 CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA 1861 AAAATCACGG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG 1921 GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC 1981 TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT 2041 GCCCGCCTGA TGAACGCTCA CCCGGAGTTT CGTATGGCCA TGAAAGACGG TGAGCTGGTG 2101 ATCTGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCG 2161 TCCCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TCCACATATA TTCGCAAGAT 2221 GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT 2281 TTTGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG 2341 GACAACTTCT TCGCCCCCGT TTTCACGATG GGCAAATATT ATACGCAAGG CGACAAGGTG 2401 CTGATGCCGC TGGCGATCCA GGTTCATCAT GCCGTTTGTG ATGGCTTCCA TGTCGGCCGC 2461 ATGCTTAATG AATTACAACA GTACTGTGAT GAGTGGCAGG GCGGGGCGTA ATAATACTAG 2521 CTCCGGCAAA AAAACGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG 2581 ATTACTTCGC GTTTGCCACC TGACGTCTAA GAAAAGGAAT ATTCAGCAAT TTGCCCGTGC 2641 CGAAGAAAGG CCCACCCGTG AAGGTGAGCC AGTGAGTTGA TTGCTACGTA ATTAGTTAGT 2701 TAGCCCTTAG TGACTCGAAT TCGCGGCCGC TTCTAGAGCA ATACGCAAAC CGCCTCTCCC 2761 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 2821 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 2881 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACATA 2941 CTAGAGAAAG AGGAGAAATA CTAGATGTCC GAGCCGGAAG AGCAGCAGCC CGACATCCAC 3001 ACGACCGCGG GCAAGCTCGC GGATCTCAGG CGCCGTATCG AGGAAGCGAC GCACGCCGGT 3061 TCCGCACGCG CCGTCGAGAA GCAGCACGCC AAGGGCAAGC TGACGGCTCG TGAACGCATC 3121 GACCTCCTCC TCGACGAGGG TTCCTTCGTC GAGCTGGACG AGTTCGCCCG GCACCGCTCC 3181 ACCAACTTCG GCCTCGACGC CAACCGCCCC TACGGCGACG GCGTCGTCAC CGGCTACGGC 3241 ACCGTCGACG GCCGCCCCGT GGCCGTCTTC TCCCAGGACT TCACCGTCTT CGGCGGCGCG 3301 CTGGGCGAGG TCTACGGCCA GAAGATCGTC AAGGTGATGG ACTTCGCCCT CAAGACCGGC 3361 TGCCCGGTCG TCGGCATCAA CGACTCCGGC GGCGCCCGCA TCCAGGAGGG CGTGGCCTCC 3421 CTCGGCGCCT ACGGCGAGAT CTTCCGCCGC AACACCCACG CCTCCGGCGT GATCCCGCAG 3481 ATCAGCCTGG TCGTCGGCCC GTGTGCGGGC GGCGCGGTGT ACTCCCCCGC GATCACCGAC 3541 TTCACGGTGA TGGTGGACCA GACCAGCCAC ATGTTCATCA CCGGTCCCGA CGTCATCAAG 3601 ACGGTCACCG GCGAGGACGT CGGCTTCGAG GAGCTGGGCG GCGCCCGCAC CCACAACTCC 3661 ACCTCGGGCG TGGCCCACCA CATGGCCGGC GACGAGAAGG ACGCGGTCGA GTACGTCAAG 3721 CAGCTCCTGT CGTACCTGCC GTCCAACAAC CTCTCCGAGC CCCCCGCCTT CCCGGAGGAG 3781 GCGGACCTCG CGGTCACGGA CGAGGACGCC GAGCTGGACA CGATCGTCCC GGACTCGGCG 3841 AACCAGCCCT ACGACATGCA CTCCGTCATC GAGCACGTCC TGGACGACGC CGAGTTCTTC 3901 GAGACGCAAC CCCTCTTCGC GCCGAACATC CTCACCGGCT TCGGCCGCGT GGAGGGCCGC 3961 CCGGTCGGCA TCGTCGCCAA CCAGCCCATG CAGTTCGCCG GCTGCCTGGA CATCACGGCC 4021 TCCGAGAAGG CGGCCCGCTT CGTGCGCACC TGCGACGCCT TCAACGTCCC CGTCCTCACC 4081 TTCGTGGACG TCCCCGGCTT CCTGCCCGGC GTCGACCAGG AGCACGACGG CATCATCCGC 4141 CGCGGCGCCA AGCTGATCTT CGCCTACGCC GAGGCCACGG TGCCGCTCAT CACGGTCATC 4201 ACCCGCAAGG CCTTCGGCGG CGCCTACGAC GTCATGGGCT CCAAGCACCT GGGCGCCGAC 4261 CTCAACCTGG CCTGGCCCAC CGCCCAGATC GCCGTCATGG GCGCCCAAGG CGCGGTCAAC 4321 ATCCTGCACC GCCGCACCAT CGCCGACGCC GGTGACGACG CCGAGGCCAC CCGGGCCCGC 4381 CTGATCCAGG AGTACGAGGA CGCCCTCCTC AACCCCTACA CGGCGGCCGA ACGCGGCTAC 4441 GTCGACGCCG TGATCATGCC CTCCGACACT CGCCGCCACA TCGTCCGCGG CCTGCGCCAG 4501 CTGCGCACCA AGCGCGAGTC CCTGCCCCCG AAGAAGCACG GCAACATCCC CCTGTGAATG 4561 CATGAGGGAG CCTCAATCGT GCGCAAGGTG CTCATCGCCA ATCGTGGCGA AATCGCTGTC 4621 CGCGTGGCCC GGGCCTGCCG GGACGCCGGG ATCGCGAGCG TGGCCGTCTA CGCGGATCCG 4681 GACCGGGACG CGTTGCACGT CCGTGCCGCT GATGAGGCGT TCGCCCTGGG TGGTGACACC 4741 CCCGCGACCA GCTATCTGGA CATCGCCAAG GTCCTCAAAG CCGCGCGCGA GTCGGGCGCG 4801 GACGCCATCC ACCCCGGCTA CGGGTTCCTC TCGGAGAACG CCGAGTTCGC GCAGGCGGTC 4861 CTGGACGCCG GCCTGATCTG GATCGGCCCG CCCCCGCACG CCATCCGCGA CCTGGGCGAC 4921 AAGGTCGCCG CCCGCCACAT CGCCCAGCGG GCCGGCGCCC CCCTGGTCGC CGGCACCCCC 4981 GACCCCGTCT CCGGCGCGGA CGAGGTCGTC GCCTTCGCCA AGGAGCACGG CCTGCCCATC 5041 GCCATCAAGG CCGCCTTCGG CGGCGGCGGG CGCGGCCTCA AGGTCGCCCG CACCCTCGAA 5101 GAGGTGCCGG AGCTGTACGA CTCCGCCGTC CGCGAGGCCG TGGCCGCCTT CGGCCGCGGG 5161 GAGTGCTTCG TCGAGCGCTA CCTCGACAAG CCCCGCCACG TGGAGACCCA GTGCCTGGCC 5221 GACACCCACG GCAACGTGGT CGTCGTCTCC ACCCGCGACT GCTCCCTCCA GCGCCGCCAC 5281 CAAAAGCTCG TCGAGGAGGC CCCCGCGCCC TTCCTCTCCG AGGCCCAGAC GGAGCAGCTG 5341 TACTCATCCT CCAAGGCCAT CCTGAAGGAG GCCGGCTACG TCGGCGCCGG CACCGTGGAG 5401 TTCCTCGTCG GCATGGACGG CACGATCTCC TTCCTGGAGG TCAACACCCG CCTCCAGGTC 5461 GAGCACCCGG TCACCGAGGA AGTCGCCGGC ATCGACCTGG TCCGCGAGAT GTTCCGCATC 5521 GCCGACGGCG AGGAACTCGG CTACGACGAC CCCGCCCTGC GCGGCCACTC CTTCGAGTTC 5581 CGCATCAACG GCGAGGACCC CGGCCGCGGC TTCCTGCCCG CCCCCGGCAC CGTCACCCTC 5641 TTCGACGCGC CCACCGGCCC CGGCGTCCGC CTGGACGCCG GCGTCGAGTC CGGCTCCGTC 5701 ATCGGCCCCG CCTGGGACTC CCTCCTCGCC AAACTGATCG TCACCGGCCG CACCCGCGCC 5761 GAGGCACTCC AGCGCGCGGC CCGCGCCCTG GACGAGTTCA CCGTCGAGGG CATGGCCACC 5821 GCCATCCCCT TCCACCGCAC GGTCGTCCGC GACCCGGCCT TCGCCCCCGA ACTCACCGGC 5881 TCCACGGACC CCTTCACCGT CCACACCCGG TGGATCGAGA CGGAGTTCGT CAACGAGATC 5941 AAGCCCTTCA CCACGCCCGC CGACACCGAG ACGGACGAGG AGTCGGGCCG GGAGACGGTC 6001 GTCGTCGAGG TCGGCGGCAA GCGCCTGGAA GTCTCCCTCC CCTCCAGCCT GGGCATGTCC 6061 CTGGCCCGCA CCGGCCTGGC CGCCGGGGCC CGCCCCAAGC GCCGCGCGGC CAAGAAGTCC 6121 GGCCCCGCCG CCTCGGGCGA CACCCTCGCC TCCCCGATGC AGGGCACGAT CGTCAAGATC 6181 GCCGTCGAGG AGGGCCAGGA AGTCCAGGAA GGCGACCTCA TCGTCGTACT CGAGGCGATG 6241 AAGATGGAAC AGCCCCTCAA CGCCCACAGG TCCGGCACCA TCAAGGGCCT CACCGCCGAG 6301 GTCGGCGCCT CCCTCACCTC CGGCGCCGCC ATCTGCGAGA ATCACTAGCA ACTTCATTAA 6361 AGAGGAGAAA TACTAGATGA AGATTTACGG AATTTATATG GACCGCCCGC TTTCACAGGA 6421 AGAAAATGAA CGGTTCATGA CTTTCATATC ACCTGAAAAA CGGGAGAAAT GCCGGAGATT 6481 TTATCATAAA GAAGATGCTC ACCGCACCCT GCTGGGAGAT GTGCTCGTTC GCTCAGTCAT 6541 AAGCAGGCAG TATCAGTTGG ACAAATCCGA TATCCGCTTT AGCACGCAGG AATACGGGAA 6601 GCCGTGCATC CCTGATCTTC CCGACGCTCA TTTCAACATT TCTCACTCCG GCCGCTGGGT 6661 CATTGGTGCG TTTGATTCAC AGCCGATCGG CATAGATATC GAAAAAACGA AACCGATCAG 6721 CCTTGAGATC GCCAAGCGCT TCTTTTCAAA AACAGAGTAC AGCGACCTTT TAGCAAAAGA 6781 CAAGGACGAG CAGACAGACT ATTTTTATCA TCTATGGTCA ATGAAAGAAA GCTTTATCAA 6841 ACAGGAAGGC AAAGGCTTAT CGCTTCCGCT TGATTCCTTT TCAGTGCGCC TGCATCAGGA 6901 CGGACAAGTA TCCATTGAGC TTCCGGACAG CCATTCCCCA TGCTATATCA AAACGTATGA 6961 GGTCGATCCC GGCTACAAAA TGGCTGTATG CGCCGCACAC CCTGATTTCC CCGAGGATAT 7021 CACAATGGTC TCGTACGAAG AGCTTTTATA ATACTAGAGC CAGGCATCAA ATAAAACGAA 7081 AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCT 7141 ACTAGAGTCA CACTGGCTCA CCTTCGGGTG GGCCTTTCTG CGTTTATA //