LOCUS       pKH7-BBa_svp_Svert_CDS  2819 bp                         11-AUG-2013
UNIMARK     pKH7-BBa_svp_Svert_CDS annotations
            pKH7-BBa_svp_Svert_CDS
FEATURES             Location/Qualifiers
     source          1..2819
                     /comment="Molecule is created with Unipro UGENE v1.12"
     stem_loop       2..73
                     /label="E. coli his operon terminator"
     stem_loop       10..44
                     /label="Stem loop"
     primer_bind     complement(137..156)
                     /label="Verification reverse (VR) primer binding site"
     misc_feature    complement(241..855)
                     /gene="rep (pMB1)"
                     /ugene_name="misc"
     misc_feature    256..256
                     /gene="ORI"
                     /ugene_name="misc"
     stem_loop       1022..1127
                     /label="T0 terminator"
     CDS             complement(1140..1799)
                     /gene="Chloramphenicol resistance"
     primer_bind     1913..1932
                     /label="Verification forward (VF2) primer binding site"
     stem_loop       complement(1989..1992)
                     /label="end of terminator"
     stem_loop       complement(2002..2021)
                     /label="Stem loop"
     misc_feature    2029..2072
                     /mismatches=0
                     /ugene_name="KH17_fw"
                     /ugene_group="Primer"
     misc_feature    2049..2792
                     /mismatches=0
                     /ugene_name="svp-Svert"
     misc_feature    complement(2781..2799)
                     /mismatches=0
                     /ugene_name="test"
     misc_feature    complement(join(2781..2819,1..1))
                     /mismatches=0
                     /ugene_name="KH18_rv"
                     /ugene_group="Primer"
ORIGIN
        1 GTCCGGCAAA AAAGGGCAAG GTGTCACCAC CCTGCCCTTT TTCTTTAAAA CCGAAAAGAT
       61 TACTTCGCGT TATGCAGGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
      121 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
      181 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
      241 CGTTGCTGGC GTTTTTCCAC AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
      301 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
      361 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
      421 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
      481 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
      541 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
      601 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
      661 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
      721 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
      781 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
      841 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
      901 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
      961 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC
     1021 TTGGATTCTC ACCAATAAAA AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT
     1081 GGAGTTCTGA GGTCATTACT GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT
     1141 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA
     1201 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG
     1261 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC
     1321 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC
     1381 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA
     1441 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT
     1501 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA
     1561 CCGTCTTTCA TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA
     1621 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA
     1681 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT
     1741 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT
     1801 TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT
     1861 ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCCGA TCAACTCGAG TGCCACCTGA
     1921 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCA
     1981 GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT GATTTCTGGA ATTCGCGGCC
     2041 GCTTCTAGAT GATCGCCGCC CTCCTGCCCT CCTGGGCCGT CACCGAACAC GCCTTCACCG
     2101 ACGCCCCGGA CGACCCGGTG AGCCTCCTCT TCCCCGAGGA GGCCGCCCAC GTCGCCCGCG
     2161 CCGTCCCCAA GCGCCTGCAC GAGTTCGCCA CCGTCCGGGT GTGCGCCCGC GCCGCCCTCG
     2221 GCCGGCTGGG CCTCCCGCCC GGTCCGCTGC TGCCCGGCCG ACGGGGCGCG CCGAGCTGGC
     2281 CGGACGGGGT GGTGGGGAGC ATGACGCACT GTCAGGGCTT CCGGGGCGCC GCGGTCGCCC
     2341 GGGCCGCCGA CGCCGCGTCG CTCGGGATAG ACGCCGAGCC GAACGGGCCG CTCCCGGACG
     2401 GCGTCCTCGC CATGGTCTCG CTGCCGTCCG AGCGCGAGTG GCTCGCCGGA CTGGCGGCCC
     2461 GCCGGCCGGA CGTGCACTGG GACCGGCTGC TGTTCAGCGC CAAGGAGAGC GTCTTCAAGG
     2521 CGTGGTACCC GCTGACCGGC CTGGAGCTGG ACTTCGACGA GGCCGAGCTG GCCGTCGATC
     2581 CGGACGCCGG GACGTTCACG GCCCGGCTGC TGGTGCCGGG ACCGGTGGTC GGCGGCCGTC
     2641 GGCTGGACGG GTTCGAGGGG CGCTGGGCGG CGGGCGAGGG CCTCGTCGTC ACGGCCATCG
     2701 CCGTCGCGGC GCCGGCCGGT ACCGCGGAGG AATCGGCGGA AGGGGCCGGG AAGGAAGCGA
     2761 CTGCGGACGA CCGGACCGCC GTCCCGTAAT GAGCTAGCTT ACTAGTAGCG GCCGCTGCA
//
