LOCUS       pRB18                   3846 bp                         05-SEP-2013
UNIMARK     pRB18_Annotations
            pRB18
FEATURES             Location/Qualifiers
     source          1..3846
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    2..73
                     /gene="E. coli his operon terminator"
                     /ugene_name="misc"
     stem_loop       10..44
                     /label="terminator stem loop"
     misc_feature    14..15
                     /gene="deletion of two bases"
                     /ugene_name="misc"
     primer_bind     complement(137..156)
                     /label="VR"
     CDS             complement(219..1034)
                     /gene="kanR (rev)"
     CDS             complement(1366..2190)
                     /gene="p15A ORI (rev)"
     primer_bind     2591..2610
                     /label="VF2"
     misc_feature    complement(2667..2670)
                     /gene="end of terminator"
                     /ugene_name="misc"
     stem_loop       complement(2680..2699)
                     /label="terminator stem loop"
     misc_feature    2731..2930
                     /label="BBa_R0010"
     misc_binding    2819..2856
                     /label="CAP"
     promoter        2867..2872
                     /label=-35
     promoter        2891..2896
                     /label=-10
     misc_binding    2896..2930
                     /label="LacI"
     misc_feature    2903..2903
                     /label="start: "
     misc_feature    complement(2913..2962)
                     /mismatches=0
                     /ugene_name="RB63_rv"
                     /ugene_group="Primer"
     misc_feature    2943..2983
                     /mismatches=0
                     /ugene_name="RB66_fw"
                     /ugene_group="Primer"
     misc_feature    2960..3649
                     /mismatches=0
                     /ugene_name="delC"
                     /ugene_group="gene"
     misc_feature    complement(3625..3669)
                     /mismatches=0
                     /ugene_name="RB67_rv"
                     /ugene_group="Primer"
     misc_feature    3647..3649
                     /ugene_name="mismatch_quest"
                     /ugene_group="misc_feature"
     misc_feature    3659..3681
                     /mismatches=0
                     /ugene_name="RB21_fw"
                     /ugene_group="Primer_p"
     misc_feature    3670..3669
                     /label="BBa_E1010"
     misc_feature    3670..3689
                     /label="barcode: "
     misc_feature    3698..3777
                     /label="BBa_B0010"
     stem_loop       3709..3752
     misc_feature    3786..3826
                     /label="BBa_B0012"
     stem_loop       3793..3812
                     /label="T7 TE"
     polyA_signal    3813..3826
     misc_feature    3819..3819
                     /label="stop: "
ORIGIN
        1 GTCCGGCAAA AAAACGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG
       61 ATTACTTCGC GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATCTCG AGTCCCGTCA AGTCAGCGTA
      181 ATGCTCTGCC AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC
      241 AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT
      301 TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT
      361 CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA
      421 ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA
      481 AGCTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA
      541 TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG
      601 CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT
      661 GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT
      721 GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC
      781 TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA
      841 ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC
      901 CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC
      961 CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT
     1021 TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT
     1081 CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG
     1141 CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC
     1201 AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC
     1261 AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC
     1321 TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTAGCG GAGTGTATAC
     1381 TGGCTTACTA TGTTGGCACT GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA
     1441 AAAGGCTGCA CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT
     1501 CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT ACGAACGGGG
     1561 CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG GAAGTGAGAG GGCCGCGGCA
     1621 AAGCCGTTTT TCCATAGGCT CCGCCCCCCT GACAAGCATC ACGAAATCTG ACGCTCAAAT
     1681 CAGTGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCT GGCGGCTCCC
     1741 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1801 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1861 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1921 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1981 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     2041 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     2101 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     2161 AAAACGATCT CAAGAAGATC ATCTTATTAA GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     2221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     2281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     2341 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     2401 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     2461 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2521 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2581 TATTCCATGG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     2641 AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT
     2701 GATTTCTGGA ATTCGCGGCC GCTTCTAGAG CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     2761 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     2821 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
     2881 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA TACTAGAGTT
     2941 CACACAGGAA ACCGGATCCA TGCAGCTCGT GTCCGTGCGT GAGGCCATGA GCCAGGGCAT
     3001 CGAGGTCTTC AGGCTGGACC TGGACCTGTC GGCCGAACTG AGCGACAGCC TGCCGCACCT
     3061 GACCCCCGAG GAGCGCGGTC GCGCCGCGCG CTTCGTGCGG GCCGCCGACC GGCTGCGCTT
     3121 CGCACAGACA CGCGCTGCCA CGCGGCGGCT GCTGGGGCGC AGGCTGGGCT GCTGCCCGGC
     3181 AGATGTGCCG CTGGCGGTCG GCGTCCACGG CAAGCCCTTT GTCGACGAGG ACCCGCTGCG
     3241 GGCACCGCTC TTCAATGTCT CGCATTCGGG CGGGCATGCG CTGATCGCGC TGGCCGATCC
     3301 CGGTGGCGTG CTGCACCTGG GCGTGGACAT CGAGCAGCAC AAGCCCGACC TCGATGCCGA
     3361 GGTCATGCTC GACGCCGTCT GCACCGACCA GGAACGCGCC AGCGTGCGCC GTGCGCCTGA
     3421 TCGGCTGGCG GCCTTCTACC AGCGCTGGGT GGGCAAGGAA GCCGTGCTCA AGGCCATCGG
     3481 CATCGGCGTG GCCGACCACC TGCGCTCCAT AGGCATCAGC CCGGGAGGGG ACGGACGGCT
     3541 GGCCATCGCC TGCGCGGTCC CCGACTGGAA TGGTTTTCAA GCGATGGCGC TGCCAGCGCC
     3601 GCCGGGCTAC GCGGCAGCTC TTGCGTGGCG CACCAAGGAA TCGACATGAT GAGCTAGCTA
     3661 ATAACGCTGA TAGTGCTAGT GTAGATCGCT ACTAGAGCCA GGCATCAAAT AAAACGAAAG
     3721 GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC
     3781 TAGAGTCACA CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATATACT AGTAGCGGCC
     3841 GCTGCA
//
