LOCUS       pRB16                   3897 bp    CIRCULAR             05-SEP-2013
EMBL_ID     pRB16                   3897 bp                         05-SEP-2013
UNIMARK     pRB16_Annotations
            pRB16
FEATURES             Location/Qualifiers
     source          1..3897
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    2..73
                     /gene="E. coli his operon terminator"
                     /ugene_name="misc"
     stem_loop       10..44
                     /label="terminator stem loop"
     misc_feature    14..15
                     /gene="deletion of two bases"
                     /ugene_name="misc"
     primer_bind     complement(137..156)
                     /label="VR"
     CDS             complement(219..1034)
                     /gene="kanR (rev)"
     CDS             complement(1366..2190)
                     /gene="p15A ORI (rev)"
     primer_bind     2591..2610
                     /label="VF2"
     misc_feature    complement(2667..2670)
                     /gene="end of terminator"
                     /ugene_name="misc"
     stem_loop       complement(2680..2699)
                     /label="terminator stem loop"
     misc_feature    2731..2930
                     /label="BBa_R0010"
     misc_binding    2819..2856
                     /label="CAP"
     promoter        2867..2872
                     /label=-35
     promoter        2891..2896
                     /label=-10
     misc_binding    2896..2930
                     /label="LacI"
     misc_feature    2903..2903
                     /label="start: "
     misc_feature    complement(2913..2962)
                     /mismatches=0
                     /ugene_name="RB63_rv"
                     /ugene_group="Primer"
     misc_feature    2943..2974
                     /mismatches=0
                     /ugene_name="RB29_fw"
                     /ugene_group="Primer"
     misc_feature    2960..3703
                     /mismatches=0
                     /ugene_name="svp"
                     /ugene_group="gene"
     misc_feature    complement(3685..3720)
                     /mismatches=0
                     /ugene_name="RB30_rv"
                     /ugene_group="Primer"
     misc_feature    3698..3732
                     /mismatches=0
                     /ugene_name="RB21_fw"
                     /ugene_group="Primer"
     misc_feature    3721..3740
                     /label="barcode: "
     misc_feature    3749..3828
                     /label="BBa_B0010"
     stem_loop       3760..3803
     misc_feature    3837..3877
                     /label="BBa_B0012"
     stem_loop       3844..3863
                     /label="T7 TE"
     polyA_signal    3864..3877
     misc_feature    3870..3870
                     /label="stop: "
ORIGIN
        1 GTCCGGCAAA AAAACGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG
       61 ATTACTTCGC GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATCTCG AGTCCCGTCA AGTCAGCGTA
      181 ATGCTCTGCC AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC
      241 AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT
      301 TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT
      361 CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA
      421 ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA
      481 AGCTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA
      541 TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG
      601 CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT
      661 GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT
      721 GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC
      781 TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA
      841 ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC
      901 CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC
      961 CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT
     1021 TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT
     1081 CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG
     1141 CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC
     1201 AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC
     1261 AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC
     1321 TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTAGCG GAGTGTATAC
     1381 TGGCTTACTA TGTTGGCACT GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA
     1441 AAAGGCTGCA CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT
     1501 CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT ACGAACGGGG
     1561 CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG GAAGTGAGAG GGCCGCGGCA
     1621 AAGCCGTTTT TCCATAGGCT CCGCCCCCCT GACAAGCATC ACGAAATCTG ACGCTCAAAT
     1681 CAGTGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCT GGCGGCTCCC
     1741 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1801 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1861 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1921 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1981 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     2041 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     2101 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     2161 AAAACGATCT CAAGAAGATC ATCTTATTAA GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     2221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     2281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     2341 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     2401 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     2461 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2521 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2581 TATTCCATGG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     2641 AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT
     2701 GATTTCTGGA ATTCGCGGCC GCTTCTAGAG CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     2761 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     2821 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
     2881 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA TACTAGAGTT
     2941 CACACAGGAA ACCGGATCCA TGATCGCCGC CCTCCTGCCC TCCTGGGCCG TCACCGAACA
     3001 CGCCTTCACC GACGCCCCGG ACGACCCGGT GAGCCTCCTC TTCCCCGAGG AGGCCGCCCA
     3061 CGTCGCCCGC GCCGTCCCCA AGCGCCTGCA CGAGTTCGCC ACCGTCCGGG TGTGCGCCCG
     3121 CGCCGCCCTC GGCCGGCTGG GCCTCCCGCC CGGTCCGCTG CTGCCCGGCC GACGGGGCGC
     3181 GCCGAGCTGG CCGGACGGGG TGGTGGGGAG CATGACGCAC TGTCAGGGCT TCCGGGGCGC
     3241 CGCGGTCGCC CGGGCCGCCG ACGCCGCGTC GCTCGGGATA GACGCCGAGC CGAACGGGCC
     3301 GCTCCCGGAC GGCGTCCTCG CCATGGTCTC GCTGCCGTCC GAGCGCGAGT GGCTCGCCGG
     3361 ACTGGCGGCC CGCCGGCCGG ACGTGCACTG GGACCGGCTG CTGTTCAGCG CCAAGGAGAG
     3421 CGTCTTCAAG GCGTGGTACC CGCTGACCGG CCTGGAGCTG GACTTCGACG AGGCCGAGCT
     3481 GGCCGTCGAT CCGGACGCCG GGACGTTCAC GGCCCGGCTG CTGGTGCCGG GACCGGTGGT
     3541 CGGCGGCCGT CGGCTGGACG GGTTCGAGGG GCGCTGGGCG GCGGGCGAGG GCCTCGTCGT
     3601 CACGGCCATC GCCGTCGCGG CGCCGGCCGG TACCGCGGAG GAATCGGCGG AAGGGGCCGG
     3661 GAAGGAAGCG ACTGCGGACG ACCGGACCGC CGTCCCGTAA TGAGCTAGCT AATAACGCTG
     3721 ATAGTGCTAG TGTAGATCGC TACTAGAGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG
     3781 AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCTA CTAGAGTCAC
     3841 ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATATAC TAGTAGCGGC CGCTGCA
//
