LOCUS       pSB6A1_BBa_J23114_BBa_B0032_ccdB_cassette 4837 bp       27-SEP-2013
EMBL_ID     pSB6A1_BBa_J23114_BBa_B0032_ccdB_cassette 4841 bp       27-SEP-2013
EMBL_ID     pSB6A1_BBa_J23114_BBa_B0032_ccdB_cassette 4841 bp  CIRCULAR  27-SEP-2013
UNIMARK     pSB6A1_BBa_J23114_BBa_B0032_ccdB_cassette annotations
            pSB6A1_BBa_J23114_BBa_B0032_ccdB_cassette
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /mismatches=0
     misc_feature    1..4098
                     /ugene_name="pSB6A1"
                     /ugene_group="Backbone"
     misc_feature    1..23
                     /ugene_name="FS_94_fw"
                     /ugene_group="Primer"
     misc_feature    36..76
                     /ugene_name="Terminator_BB0012"
     misc_feature    complement(440..460)
                     /Primer_screening="FS_76_SR_06_rv"
                     /ugene_name="Primer_screening"
     misc_feature    3750..3769
                     /Primer_screening="FS_72_SR_02_fw"
                     /ugene_name="Primer_screening"
     misc_feature    3994..4013
                     /ugene_name="VF2_primer"
     misc_feature    complement(4077..4128)
                     /ugene_name="FS_85_order_rv"
                     /ugene_group="Primer"
     misc_feature    4099..4133
                     /ugene_name="BBa_J23114"
     misc_feature    4110..4179
                     /ugene_name="FS_90_fw"
                     /ugene_group="Primer"
     misc_feature    complement(4114..4179)
                     /ugene_name="FS_88_rv_1"
                     /ugene_group="Primer"
     misc_feature    complement(4114..4185)
                     /ugene_name="FS_88_rv_2"
                     /ugene_group="Primer"
     misc_feature    4134..4155
                     /ugene_name="BBa_B0032"
     misc_feature    4156..4159
                     /ugene_name="KpnI"
     misc_feature    4160..4833
                     /score=3343
                     /ugene_name="ccdB_cassette"
     misc_feature    4160..4185
                     /ugene_name="FS_89_fw"
                     /ugene_group="Primer"
     misc_feature    complement(4187..4586)
                     /mismatches=67
                     /ugene_name="P1011"
     misc_feature    complement(4191..4493)
                     /mismatches=0
                     /ugene_name="ccdb"
     misc_feature    complement(join(4816..4837,1..21))
                     /ugene_name="FS_93_rv"
                     /ugene_group="Primer"
     misc_feature    4834..4837
                     /ugene_name="BamHI"
ORIGIN
        1 ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG
       61 CCTTTCTGCG TTTATATACT AGTAGCGGCC GCTGCAGCTC TACGCCGGAC GCATCGTGGC
      121 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
      181 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
      241 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
      301 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
      361 GGGAGAGCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC
      421 GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG
      481 ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC
      541 GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT
      601 CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC
      661 CGACGCGCTG GGCTACGTCT TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCAT
      721 TATGATTCTT CTCGCTTCCG GCGGCATCGG GATGCCCGCG TTGCAGGCCA TGCTGTCCAG
      781 GCAGGTAGAT GACGACCATC AGGGACAGCT TCAAGGATCG CTCGCGGCTC TTACCAGCCT
      841 AACTTCGATC ACTGGACCGC TGATCGTCAC GGCGATTTAT GCCGCCTCGG CGAGCACATG
      901 GAACGGGTTG GCATGGATTG TAGGCGCCGC CCTATACCTT GTCTGCCTCC CCGCGTTGCG
      961 TCGCGGTGCA TGGAGCCGGG CCACCTCGAC CTGAATGGAA GCCGGCGGCA CCTCGCTAAC
     1021 GGATTCACCA CTCCAAGAAT TGGAGCCAAT CAATTCTTGC GGAGAACTGT GAATGCGCAA
     1081 ACCAACCCTT GGCAGAACAT ATCCATCGCG TCCGCCATCT CCAGCAGCCG CACGCGGCGC
     1141 ATCTCGGGCA GCGTTGGGTC CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG
     1201 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
     1261 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC
     1321 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG
     1381 TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA
     1441 ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC
     1501 AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC
     1561 GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
     1621 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA
     1681 AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC
     1741 ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC
     1801 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     1861 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     1921 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG
     1981 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT
     2041 GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
     2101 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     2161 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     2221 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     2281 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     2341 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     2401 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     2461 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     2521 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     2581 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     2641 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
     2701 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     2761 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     2821 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     2881 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
     2941 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
     3001 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
     3061 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
     3121 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
     3181 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
     3241 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
     3301 GTTTGCGCAA CGTTGTTGCC ATTGCTGTAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
     3361 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
     3421 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
     3481 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
     3541 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
     3601 GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT AGCAGAACTT
     3661 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
     3721 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
     3781 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
     3841 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
     3901 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
     3961 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA
     4021 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAAGAAT
     4081 TCGCGGCCGC TTCTAGAGTT TATGGCTAGC TCAGTCCTAG GTACAATGCT AGCTCTAGAG
     4141 TCACACAGGA AAGCAGTACA CTGGCTGTGT ATAAGGGAGC CTGACATTTA TATTCCCCAG
     4201 AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC AGCCACTTCT
     4261 TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG CCAGCTTTCA
     4321 TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG CAGACGTGCA
     4381 CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT CTGTACATCC
     4441 ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG CATTTCACCA
     4501 GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA CCTCAGCCAT
     4561 CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT CATTCTGCAT
     4621 GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA CTGATAGCTG
     4681 TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT GACATACTTC
     4741 GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA TACGCATACT
     4801 GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGGATC
//
