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<p>1>Bacteria must be kept in the -80’c circumstance dissolved in the glycerol, and the content of the glycerol is roughly 20% to 30%.<br> 　　　Materials: 40% glycerol, bacteria in the fluid culture.</p> <p>2>Absorb 600ul bacteria solution and 500ul 40% glycerol, and inject them into the 1.5ml EP tube.</p> <p>3>Use the marker pen to put down the specific type and relevant resistance of the bacteria, then seal the tube and preserve in the -80’c.</p>

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