Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Exp/10.16CsClGradient

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification September - October Notebook: Experiments'''</font>

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: <u> '''Phage Purification''' </u> </font>

: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]

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<font size="5"> '''10.16 T7 EM''' </font>

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'''I) Purpose'''
: Identify and measure capsid sizes of mutated phage.

'''II) Expected Outcome'''
: Small and large mutated phage.

'''III) Reagants Used'''
: T7 mutant phage
: TEM kit


'''IV) Actual Procedure'''

: Place 10 microL of sample onto EM grid. Let sit for 1 min.
: Dry by touching a dry piece of material to the side of the grid. (provided in EM kit).
: Place 10 microL EM dye onto grid. Wait 90 seconds before drying.
: Place in EM grid carrier. Deliver to the TEM operator for imaging.

'''V) Results'''
:  Found 3 smaller mutants and 1 large mutant. We will take more pictures to measure more capsid sizes.
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[[File:T7_1st_Gradient_09.11.13.jpg | thumb|none|alt=A|T7 First Gradient]]
[[File:T7_2nd_Gradient_09.11.13.jpg | thumb|none|alt=A|T7 Second Gradient]]
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