Team:Sumbawagen

<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="ja" lang="ja">
  <head>


    <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
    <meta name="Generator" content="iWeb 3.0.4" />
    <meta name="iWeb-Build" content="local-build-20130925" />
    <meta http-equiv="X-UA-Compatible" content="IE=EmulateIE7" />
    <meta name="viewport" content="width=700" />
    <title>Sumbawagen iGEM Team</title>
    <link rel="stylesheet" type="text/css" media="screen,print" href="http://static.tumblr.com/jufbrt5/GUxmtoho6/human_practice.css" />
    <!--[if lt IE 8]><link rel='stylesheet' type='text/css' media='screen,print' href='http://static.tumblr.com/jufbrt5/QESmtoj1z/human_practiceie.css'/><![endif]-->
    <!--[if gte IE 8]><link rel='stylesheet' type='text/css' media='screen,print' href='Media/IE8.css'/><![endif]-->
    <script type="text/javascript" src="http://static.tumblr.com/jufbrt5/wgFmtok64/iwebsite.js"></script>
    <script type="text/javascript" src="http://static.tumblr.com/jufbrt5/DuCmtok8e/widgetcommon.js"></script>
    <script type="text/javascript" src="http://static.tumblr.com/jufbrt5/Yigmtok9c/navbar.js"></script>
    <script type="text/javascript" src="http://static.tumblr.com/jufbrt5/f84mtoizn/human_practice.js"></script>

<style type ='text/css'>

#globalWrapper {
width: 1410px;
height:100%;
}

#content {
background-color: transparent;
border: none;
padding: 0;
margin: 0;
margin-top: -23px;
width: 100%;
overflow: hidden;
height:100%;
}

#bodyContent {
border: none;
padding:0;
margin:0;
width:1410px;
height:100%;
}

#top-section {
height: 18px;
margin: 0px;
margin-top: 5px;
margin-left: auto;
margin-right: auto;
margin-bottom: 0 !important;
padding:0;
border: none;
font-size: 10px;
}

#p-logo {
overflow:hidden;
display: none;
}

#search-controls {
display:none;
}

#footer-box {
width: 1410px;
height: ;
}

#footer {
border: none;
width: 1410px;
}

.firstHeading {
display: none;
}

#f-list a {
color: #333;
font-size: 10px;
}

#f-list a:hover {
color: #666;
}

.printfooter {
display: none;
}

h3#siteSub {
display: none;
}

#contentSub {
display: none;
}


#menu{ 

width:400px;

}



</style>
  

</head>
  <body style="background: rgb(255, 255, 255) url(https://static.igem.org/mediawiki/2013/4/4b/Playtime_background_yellow.jpg) repeat scroll top left; margin: 0pt; " onload="onPageLoad();" onunload="onPageUnload();">
    <div style="text-align: center; ">
      <div style="margin-bottom: 15px; margin-left: auto; margin-right: auto; margin-top: 15px; overflow: hidden; position: relative; word-wrap: break-word;  text-align: left; width: 700px; " id="body_content">
        <div style="background: transparent url(https://static.igem.org/mediawiki/2013/6/65/Playtime_page_bg-6.png) repeat scroll top left; width: 700px; ">
          <div style="height: 111px; margin-left: 0px; position: relative; width: 700px; z-index: 10; " id="header_layer">
            <div style="height: 0px; line-height: 0px; " class="bumper"> </div>
            <div id="id1" style="height: 61px; left: 22px; position: absolute; top: 50px; width: 645px; z-index: 1; " class="style_SkipStroke shape-with-text">
              <div class="text-content style_External_645_61" style="padding: 0px; ">
                <div class="style">
                  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="paragraph_style">Sumbawagen iGEM Team</p>
                </div>
              </div>
            </div>
            


            <div style="height: 22px; width: 645px;  height: 22px; left: 22px; position: absolute; top: 29px; width: 645px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/9/98/Kids_green_header_01.png" alt="" style="border: none; height: 22px; width: 645px; " />
            </div>
            
            <div style="height: 22px; width: 645px;  height: 22px; left: 22px; position: absolute; top: 50px; width: 645px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/4/40/Kids_green_header_02_1.png" alt="" style="border: none; height: 86px; width: 645px; " />
            </div>


            <div style="height: 20px; width: 700px;  height: 20px; left: 0px; position: absolute; top: 0px; width: 700px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/3/36/Playtime_header_bg.jpg" alt="" style="border: none; height: 20px; width: 700px; " />
            </div>
            


            <div style="height: 172px; width: 190px;  height: 172px; left: 495px; position: absolute; top: 15px; width: 190px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/3/3a/Playtime_Sun-4.png" alt="" style="border: none; height: 172px; width: 190px; " />
            </div>
<div style="height: 80px; width: 80px;  height: 80px; left: 10px; position: absolute; top: 15px; width: 190px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <a href="https://2013.igem.org/Main_Page"><img src="http://upload.wikimedia.org/wikipedia/en/d/d6/IGEM_official_logo.png" alt="" style="border: none; height: 80px; width: 90px; " />
            </a></div>

          </div>
          <div style="margin-left: 0px; position: relative; width: 700px; z-index: 0; " id="nav_layer">
            <div style="height: 0px; line-height: 0px; " class="bumper"> </div>
            <div style="height: 1px; line-height: 1px; " class="tinyText"> </div>
            <div class="com-apple-iweb-widget-navbar flowDefining" id="widget0" style="margin-left: 22px; margin-top: -1px; opacity: 1.00; position: relative; width: 645px; z-index: 1; ">
    
              <div id="widget0-navbar" class="navbar">

      
                <div id="widget0-bg" class="navbar-bg">

        
                  <ul id="widget0-navbar-list" class="navbar-list">
 <li></li> 
</ul>
                  
      
</div>
                

<div id="menu">

Home
<A Href="https://2013.igem.org/Team:Sumbawagen/Team">Team</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Modeling">Modeling</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Project">Project</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Project">Parts</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Notebook">Notebook</A>
<br>
<A Href="https://2013.igem.org/Team:Sumbawagen/Lab Preparation team activities">Lab Preparation team activities</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Safety">Safety</A>
<A Href="https://2013.igem.org/Team:Sumbawagen/Acknowledgement">Acknowledgement</A>


</div>
    
</div>
            </div>

            <div style="clear: both; height: 0px; line-height: 0px; " class="spacer"> </div>
          </div>
          <div style="margin-left: 0px; position: relative; width: 700px; z-index: 5; " id="body_layer">
            <div style="height: 0px; line-height: 0px; " class="bumper"> </div>
            <div id="id2" style="height: 34px; left: 35px; position: absolute; top: 17px; width: 620px; z-index: 1; " class="style_SkipStroke_2 shape-with-text">
              <div class="text-content Normal_External_620_34" style="padding: 0px; ">
                <div class="Normal">
                  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Title">Welcome to our wiki!</p>
                </div>
              </div>
            </div>
            


            <div style="height: 16px; width: 645px;  height: 16px; left: 22px; position: absolute; top: 0px; width: 645px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/3/3a/Kids_green_header_03.png" alt="" style="border: none; height: 16px; width: 645px; " />
            </div>
            


            <div id="id3" style="height: 2766px; left: 35px; position: absolute; top: 47px; width: 628px; z-index: 1; " class="style_SkipStroke_3 shape-with-text">
              <div class="text-content graphic_textbox_layout_style_default_External_628_2766" style="padding: 0px; ">
                <div class="graphic_textbox_layout_style_default">

<br>
<br>

<br>
<IMG SRC="https://static.igem.org/mediawiki/2013/a/a9/Home_-_aka_129.jpg" WIDTH=600 HEIGHT=300 >
<br>
<br>
E. coli which able to measure the level of sugar in honey by emitting light
<br>
Glucose and fructose are major sugar component of honey. Sumbawa honey is famous as central production of forest honey in Indonesia. Sumbawa honey is also protected as geographical indication by Indonesian patent office. Taneoka et al (2009) has created a fusion of mutant glucose binding protein and firefly luciferase, which able to measure glucose level by emitting light - intended initially for blood glucose sensor application. In this project, we plan to create this construct in Biobrick format, and evaluate the ability of transgenic E. coli for the measurement of glucose in Sumbawa honey. Our final goal is to create a device which can be used for quality control of Sumbawa honey. The basic method is rapid detection for glucose and fructose by construct BioBrick which is fussion between Glucose Binding Protein (GBP) with Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP). The BioBrick construction named "ECONEY”. 

                  




<Br>

                </div>
                <div style="clear: both; height: 0px; line-height: 0px; " class="tinyText"> </div>
              </div>
            </div>
            <div style="height: 813px; line-height: 813px; " class="spacer"> </div>
          </div>
          <div style="height: 75px; margin-left: 0px; position: relative; width: 700px; z-index: 15; " id="footer_layer">
            <div style="height: 0px; line-height: 0px; " class="bumper"> </div>
            

</a>
            <div style="height: 17px; width: 700px;  height: 17px; left: 0px; position: absolute; top: 58px; width: 700px; z-index: 1; " class="tinyText style_SkipStroke_1">
              <img src="https://static.igem.org/mediawiki/2013/b/be/Playtime_footer_bg.jpg" alt="" style="border: none; height: 17px; width: 700px; " />
            </div>
          </div>
        </div>
      </div>
    </div>
  </body>
</html>