Team:Tuebingen/Notebook/Protocols/glycerol-stocks

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Glycerol Stocks
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<h3>Procedure</h3>

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  <li>Inoculate 5 mL <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB-medium</a> with one single colony from a fresh plate (e.g. after <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/trafo">transformation</a>) and incubate at 37 °C and 220 rpm over night.</li>
  <li>On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.</li>
  <li>Add 1 mL of cell-culture and mix.</li>
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<p><a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a></p>
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