Team:Washington/96WellPlateAssay

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<b><u>96-Well Plate Assay</u></b>
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Obtain <a href = "http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=-1&productId=3349439&distype=0&fromSearch=0">96-Well Plate</a>.</br>
1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br>
2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br>
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br>
4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C.</br>
5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>

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