Team:Groningen

From 2013.igem.org

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<h1>University of Groningen</h1>
<h1>University of Groningen</h1>
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<p>We are the Igem Team for the University of Groningen.
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<p>This is the website of the iGEM team Groningen. Last year the University of Groningen did win the World Championship and we have the honor to defend that title. The members of our team are from different research fields. You can think about chemistry, biomedical engineering, physics, computing science, industrial engineering and last but not least biology. With such a variety of interest we have different perspectives into the problems that need to be solved.
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As Groningen won the world championsip last year, we are tasked with defending that honor.
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Our team consists of a good mix from diffrent research field including Chemistry, Biomedical engineering, physics, Computing Science, Industrial Engineering and of course Biology.
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A very differs team indeed and with so many different perspective we are looking forward to tackling all the different chalanges surrounding our project.
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<h2>Silk secretion By Bascillus subtilis</h2>
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<h2>Silk secretion by ''Bacillus subtilis''</h2>
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<p>The main goal of our project would be to implemt Biobricks in order to make the secretion of silk protein (spider silk as well a Bombyx mori silk) possible.
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<p> The main goal of our project is to secrete silk. The Utah 2012 team successfully produced the spider silk in ''E.coli''. So we want to use their biobrick to get the silk genes. After the production of spider silk they lysate the bacteria in order to purify the silk proteins and get the polymerization. We want to use the Sec pathway to secrete the spider silk over the cell membrane. When we succeed, it is not necessary anymore to kill the bacteria, so they can be used over and over again. The sec system is present in different organisms, but we have chosen to use ''Bacillus subtilis''. The advantage of this organims is that we can use its capability of mobility and biofilm formation.
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Also we want to devolope a new technique for spinning fibers incorporating or newly modified bacterial species.
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There are many more yet to explore application of a bacteria that can secrete silk proteins.
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As the secretion of the protein allows us to keep the bacteria alive and therefore use more of their splendid abilities.
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Revision as of 10:40, 4 June 2013


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University of Groningen

This is the website of the iGEM team Groningen. Last year the University of Groningen did win the World Championship and we have the honor to defend that title. The members of our team are from different research fields. You can think about chemistry, biomedical engineering, physics, computing science, industrial engineering and last but not least biology. With such a variety of interest we have different perspectives into the problems that need to be solved.

Silk secretion by Bacillus subtilis

The main goal of our project is to secrete silk. The Utah 2012 team successfully produced the spider silk in E.coli. So we want to use their biobrick to get the silk genes. After the production of spider silk they lysate the bacteria in order to purify the silk proteins and get the polymerization. We want to use the Sec pathway to secrete the spider silk over the cell membrane. When we succeed, it is not necessary anymore to kill the bacteria, so they can be used over and over again. The sec system is present in different organisms, but we have chosen to use Bacillus subtilis. The advantage of this organims is that we can use its capability of mobility and biofilm formation.

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