Team:Groningen

From 2013.igem.org

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<h1>University of Groningen</h1>
<h1>University of Groningen</h1>
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<p>This is the website of the iGEM team Groningen. Last year the University of Groningen won the World Championship and we have the honor of defending that title. The members of our team are from different research fields. You can think about chemistry, biomedical engineering, physics, computing science, industrial engineering and last but not least biology. With such a variety of interest we have different perspectives into the problems that we need to solve.
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<p>Welcome to the website of the 2013 iGEM team from Groningen! This year our team consists of twelve under- and postgraduate students from a wide range of disciplines; Chemistry, Biomedical Engineering, Physics, Computing Science, Industrial Engineering, Bio-informatics, and ofcourse Biology. We have a highly versatile group with many different interests, which will hopefully provide us with different perspectives on the problems to be solved.  
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<h2>Silk secretion by ''Bacillus subtilis''</h2>
<h2>Silk secretion by ''Bacillus subtilis''</h2>
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<p> The main goal of our project is to secrete silk. The Utah 2012 team successfully produced the spider silk in ''E.coli''. So we want to use their biobrick to get the silk genes. After the production of spider silk they lysate the bacteria in order to purify the silk proteins and get the polymerization. We want to use the Sec pathway to secrete the spider silk over the cell membrane. When we succeed, it is not necessary anymore to kill the bacteria, so they can be used over and over again. The sec system is present in different organisms, but we have chosen to use ''Bacillus subtilis''. The advantage of this organism is that we can use its capability of mobility and biofilm formation.
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<p> This year we will pick up where the 2012 iGEM team from Utah left off. Utah managed to successfully produce spider silk proteins in “E.Coli”, after which the bacteria was lysated to purify the silk proteins and achieve polymerization.  
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Our goal is to use these Utah's biobricks to create a silk biofilm without having to destroy the bacteria. In order to achieve this, we need to secrete the silk proteins, for which we will use the 'Sec pathway' in Bacillus Subtilis. This micro-organism is more suitable for biofilm formation than E.Coli due to it's capability of adhering to certain surfaces.  
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Revision as of 18:51, 4 June 2013


Groningen team.png


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University of Groningen

Welcome to the website of the 2013 iGEM team from Groningen! This year our team consists of twelve under- and postgraduate students from a wide range of disciplines; Chemistry, Biomedical Engineering, Physics, Computing Science, Industrial Engineering, Bio-informatics, and ofcourse Biology. We have a highly versatile group with many different interests, which will hopefully provide us with different perspectives on the problems to be solved.

Silk secretion by Bacillus subtilis

This year we will pick up where the 2012 iGEM team from Utah left off. Utah managed to successfully produce spider silk proteins in “E.Coli”, after which the bacteria was lysated to purify the silk proteins and achieve polymerization. Our goal is to use these Utah's biobricks to create a silk biofilm without having to destroy the bacteria. In order to achieve this, we need to secrete the silk proteins, for which we will use the 'Sec pathway' in Bacillus Subtilis. This micro-organism is more suitable for biofilm formation than E.Coli due to it's capability of adhering to certain surfaces.

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